ZFIN Image: Halpern et al., 1997, Fig. 3

Image

Figure Caption

Fig. 3 Expression of shh is missing in the trunk midline in cyc;flh double mutants at the late segmentation period. Whole- mounted embryos at 21 hr, left side views, with anterior to the left and dorsal to the top. RNA in situ hybridization. At this stage wild-type embryos (A) the floor plate (fp, white arrowhead) expresses shh strongly, and expression is becoming downregulated in the notochord (not, dark arrowhead), as previously shown by Krauss et al. (1993). The embryos are overstained to reveal weaker levels of notochord shh expression. In cyc mutants (B) only the notochord is labeled (Krauss et al., 1993), in flh mutants (C) only isolated clusters of floor plate cells are labeled (Talbot et al., 1995), and in cyc;flh double mutants (D), there is no labeling at all. Scale bar, 25 um.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, volume 187(2), Halpern, M.E., Hatta, K., Amacher, S.L., Talbot, W.S., Yan, Y.-L., Thisse, B., Thisse, C., Postlethwait, J.H., and Kimmel, C.B., Genetic interactions in zebrafish midline development, 154-170, Copyright 1997, with permission from Elsevier.

Reprinted from Developmental Biology, 187(2), Halpern, M.E., Hatta, K., Amacher, S.L., Talbot, W.S., Yan, Y.-L., Thisse, B., Thisse, C., Postlethwait, J.H., and Kimmel, C.B., Genetic interactions in zebrafish midline development, 154-170, Copyright (1997) with permission from Elsevier. Full text @ Dev. Biol.