A zebrafish eye paraffin section is overlaid with colors to highlight the tissues used for construction of the different cDNA libraries. In addition to the library made from whole eyes, libraries were also constructed using only lens or retinal tissue (shown in green or tan colors, respectively). The anterior segment library (purple) was made from tissues that included cornea (Co), annular ligament (AL), iris (Ir), the ciliary region (CR), and the retinal margin (CGZ), but did not include the lens. The posterior segment library (blue) consisted of retinal pigmented epithelium (RPE), choroid, sclera, and optic nerve (OpN) tissue, but not retina.
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