|
Fig. 7 A strong genetic interaction between fgf10/dae and extl3/box during limb development. Four-day live photos (A-E) of wild-type (A), fgf10/dae (B), extl3/box (C), ext2/dak (D) and box–/–;dae+/– (E) embryos. All photos are dorsal views with anterior towards the left. dae (B) mutant embryos have a severe truncation of pectoral fin compared with siblings (A). extl3/box (C) mutants have a weaker pectoral fin truncation, whereas ext2/dak (D) appears similar to fgf10/dae and has a severe truncation of the fin. Removal of one copy of fgf10 in an extl3/box mutant background (E) severely worsens the extl3/box phenotype, demonstrating a genetic interaction between dae/fgf10 and box/extl3. SNP genotyping (F) confirms the dae/fgf10 genotype (see Materials and methods). WT, wild-type embryo; het, heterozygous embryo; dae, daedalus mutant embryo; L, ladder; C, box mutant embryo shown in C; E, box–/–;dae+/– embryo shown in E.