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Fig. 7 Detection of Cre-mediated site-specific deletion by polymerase chain reaction (PCR) analysis. A: Cartoon of two expected unrecombined PCR products. B: Cartoon of expected recombined PCR product. C: Analysis of recombination in EGFP-cre Tg/+ X pCMV-loxH-STOP-loxP-EGFP reporter Tg/+ cross and confirmation of presence of EGFP-cre Tg. "H2O" refers to the water (no DNA) control PCR reaction, and "no hs" refers to genomic DNA isolated from the transgenic cross in the absence of heat shock induction.
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