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Fig. 2 Heat shock regulated expression of EGFP and EGFP-Cre. A: Mosaic transgenic zebrafish (24 hours postfertilization [hpf]) for the HSP70-EGFP construct in the absence of heat shock. B: Mosaic transgenic zebrafish (24 hpf) for the HSP70-EGFP construct heated for 90 sec at 42°C. C: Mosaic transgenic zebrafish (24 hpf) for the HSP70-EGFP-cre construct in the absence of heat shock. D: Mosaic transgenic zebrafish (24 hpf) for the HSP70-EGFP-cre construct heated for 90 sec at 42°C. E: Confocal Z-stack showing a representative HSP70-EGFP-cre Tg/Tg zebrafish at approximately 24 hpf. Brightfield images of the embryos in A-D are shown in the insets. Scale bars = 250 microns in B (applies to inset and A-D), 500 microns in E.