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Fig. 2 Expression pattern of pcdh15a using in situ hybridization. (A-E) pcdh15a expression in embryos and larvae. At the four-somite stage (A), pcdh15a is expressed in the anterior neuroectoderm and in the somites. At 24 hpf (B), there is expression in the eye, the hatching gland (blue shading under the eye, out of focus) and the first hair cells of the ear at the margins of the otic vesicle (arrowhead and panel C). At 3 dpf (D), expression of pcdh15a is not detectable in the eye, whereas expression is present in the ear (asterisk), the brain and the lateral parts of the epiphysis (arrowheads). At 4 dpf (E), expression is present in the hair cells of the ear (arrows indicate the posterior maculae present in this focal plane) and the neuromasts (arrowheads). (F) Higher magnification view of the pcdh15a-positive hair cells of the anterior macula. (F′) Higher magnification view of a trunk neuromast. Scale bar in F′: 180 µm for A,B; 50 µm for C; 120 µm for D; 150 µm for E; 30 µm for F; 20 µm for F′.