Gene
npnta
- ID
- ZDB-GENE-090312-201
- Name
- nephronectin a
- Symbol
- npnta Nomenclature History
- Previous Names
-
- npnt
- si:ch211-202n8.2
- Type
- protein_coding_gene
- Location
- Chr: 1 Mapping Details/Browsers
- Description
- Predicted to enable calcium ion binding activity. Acts upstream of or within atrioventricular valve development and heart trabecula formation. Predicted to be located in membrane. Is expressed in several structures, including digestive system; endoderm; head; heart; and tail bud. Used to study membranous glomerulonephritis and proteinuria. Orthologous to human NPNT (nephronectin).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 5 figures from 3 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
- No data available
Wild Type Expression Summary
- All Phenotype Data
- 10 figures from 3 publications
- Cross-Species Comparison
- Alliance
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| la013711Tg | Transgenic insertion | Unknown | Unknown | DNA | |
| la013712Tg | Transgenic insertion | Unknown | Unknown | DNA | |
| la013713Tg | Transgenic insertion | Unknown | Unknown | DNA | |
| la028177Tg | Transgenic insertion | Unknown | Unknown | DNA | |
| sa15583 | Allele with one point mutation | Unknown | Splice Site | ENU | |
| sa19582 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa19583 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa19584 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| zko970a | Allele with one deletion | Unknown | Unknown | CRISPR | |
| zko970b | Allele with one insertion | Unknown | Unknown | CRISPR |
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| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-npnta | China Zebrafish Resource Center (CZRC) | |
| MO1-npnta | N/A | Patra et al., 2011 |
| MO2-npnta | N/A | (3) |
| MO3-npnta | N/A | Patra et al., 2011 |
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Human Disease
| Human Disease | Fish | Conditions | Citations |
|---|---|---|---|
| proteinuria | WT + MO2-npnta | standard conditions | Müller-Deile et al., 2017 |
| membranous glomerulonephritis | WT + MO2-npnta | standard conditions | Müller-Deile et al., 2017 |
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Domain, Family, and Site Summary
Domain Details Per Protein
| Protein | Additional Resources | Length | Concanavalin A-like lectin/glucanase domain superfamily | EGF domain | EGF-like calcium-binding, conserved site | EGF-like calcium-binding domain | EGF-like domain | EGF-type aspartate/asparagine hydroxylation site | Growth factor receptor cysteine-rich domain superfamily | MAM domain | Nephronectin domain-containing protein | NOTCH1 EGF-like calcium-binding domain |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UniProtKB:A0A8M2B6Z0 | InterPro | 619 | ||||||||||
| UniProtKB:A0A8M3AVI7 | InterPro | 588 | ||||||||||
| UniProtKB:B8A5G4 | InterPro | 605 | ||||||||||
| UniProtKB:A0A8M3B3H9 | InterPro | 636 |
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Interactions and Pathways
No data available
Plasmids
No data available
No data available
| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | CH211-202N8 | ZFIN Curated Data |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_001145580 (1) | 2018 nt | ||
| Genomic | GenBank:BX537118 (1) | 197161 nt | ||
| Polypeptide | UniProtKB:A0A8M3B3H9 (1) | 636 aa |
- Müller-Deile, J., Sopel, N., Ohs, A., Rose, V., Gröner, M., Wrede, C., Hegermann, J., Daniel, C., Amann, K., Zahner, G., Schiffer, M. (2021) Glomerular Endothelial Cell-Derived microRNA-192 Regulates Nephronectin Expression in Idiopathic Membranous Glomerulonephritis. Journal of the American Society of Nephrology : JASN. 32:2777-2794
- Mukherjee, D., Wagh, G., Mokalled, M.H., Kontarakis, Z., Dickson, A.L., Rayrikar, A., Günther, S., Poss, K.D., Stainier, D.Y.R., Patra, C. (2020) Ccn2a/Ctgfa is an injury-induced matricellular factor that promotes cardiac regeneration in zebrafish. Development (Cambridge, England). 148(2):
- Müller-Deile, J., Dannenberg, J., Schroder, P., Lin, M.H., Miner, J.H., Chen, R., Bräsen, J.H., Thum, T., Nyström, J., Staggs, L.B., Haller, H., Fiedler, J., Lorenzen, J.M., Schiffer, M. (2017) Podocytes regulate the glomerular basement membrane protein nephronectin by means of miR-378a-3p in glomerular diseases. Kidney International. 92(4):836-849
- Talbot, J.C., Nichols, J.T., Yan, Y.L., Leonard, I.F., BreMiller, R.A., Amacher, S.L., Postlethwait, J.H., Kimmel, C.B. (2016) Pharyngeal morphogenesis requires fras1-itga8- dependent epithelial-mesenchymal interaction. Developmental Biology. 416(1):136-48
- Elkon, R., Milon, B., Morrison, L., Shah, M., Vijayakumar, S., Racherla, M., Leitch, C.C., Silipino, L., Hadi, S., Weiss-Gayet, M., Barras, E., Schmid, C.D., Ait-Lounis, A., Barnes, A., Song, Y., Eisenman, D.J., Eliyahu, E., Frolenkov, G.I., Strome, S.E., Durand, B., Zaghloul, N.A., Jones, S.M., Reith, W., Hertzano, R. (2015) RFX transcription factors are essential for hearing in mice. Nature communications. 6:8549
- Ho, N.Y., Yang, L., Legradi, J., Armant, O., Takamiya, M., Rastegar, S., and Strähle, U. (2013) Gene Responses in the Central Nervous System of Zebrafish Embryos Exposed to the Neurotoxicant Methyl Mercury. Environmental science & technology. 47(7):3316-25
- Varshney, G.K., Lu, J., Gildea, D., Huang, H., Pei, W., Yang, Z., Huang, S.C., Schoenfeld, D.S., Pho, N., Casero, D., Hirase, T., Mosbrook-Davis, D.M., Zhang, S., Jao, L.E., Zhang, B., Woods, I.G., Zimmerman, S., Schier, A.F., Wolfsberg, T., Pellegrini, M., Burgess, S.M., and Lin, S. (2013) A large-scale zebrafish gene knockout resource for the genome-wide study of gene function. Genome research. 23(4):727-735
- Patra, C., Diehl, F., Ferrazzi, F., van Amerongen, M.J., Novoyatleva, T., Schaefer, L., Mühlfeld, C., Jungblut, B., and Engel, F.B. (2011) Nephronectin regulates atrioventricular canal differentiation via Bmp4-Has2 signaling in zebrafish. Development (Cambridge, England). 138(20):4499-4509
- Wang, D., Jao, L.E., Zheng, N., Dolan, K., Ivey, J., Zonies, S., Wu, X., Wu, K., Yang, H., Meng, Q., Zhu, Z., Zhang, B., Lin, S., and Burgess, S.M. (2007) Efficient genome-wide mutagenesis of zebrafish genes by retroviral insertions. Proceedings of the National Academy of Sciences of the United States of America. 104(30):12428-12433
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