Gene
mcf2lb
- ID
- ZDB-GENE-081104-416
- Name
- mcf.2 cell line derived transforming sequence-like b
- Symbol
- mcf2lb Nomenclature History
- Previous Names
-
- sb:eu830
- si:dkey-46k9.10
- Type
- protein_coding_gene
- Location
- Chr: 9 Mapping Details/Browsers
- Description
- Predicted to enable guanyl-nucleotide exchange factor activity. Acts upstream of or within neuromast primordium migration. Predicted to be active in cytoplasm. Is expressed in lateral line system; otic placode; and otic vesicle. Orthologous to human MCF2L (MCF.2 cell line derived transforming sequence like).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 7 figures from 2 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
-
- eu830 (12 images)
Wild Type Expression Summary
- All Phenotype Data
- 4 figures from Olson et al., 2024
- Cross-Species Comparison
- Alliance
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| nl25 | Allele with multiple variants | Unknown | Unknown | not specified | |
| nl26 | Allele with multiple variants | Unknown | Unknown | not specified | |
| sa7189 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa15537 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa41441 | Allele with one point mutation | Unknown | Premature Stop | ENU |
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| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-mcf2lb | (2) | |
| CRISPR2-mcf2lb | (2) | |
| CRISPR3-mcf2lb | (2) |
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Human Disease
Domain, Family, and Site Summary
| Type | InterPro ID | Name |
|---|---|---|
| Conserved_site | IPR001331 | Guanine-nucleotide dissociation stimulator, CDC24, conserved site |
| Domain | IPR001251 | CRAL-TRIO lipid binding domain |
| Domain | IPR001849 | Pleckstrin homology domain |
| Domain | IPR055251 | SOS1/NGEF-like, PH domain |
| Family | IPR051336 | Rho GTPase-activating Guanine Nucleotide Exchange Factors |
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Domain Details Per Protein
| Protein | Length | CRAL-TRIO lipid binding domain | Dbl homology (DH) domain superfamily | Guanine-nucleotide dissociation stimulator, CDC24, conserved site | PH-like domain superfamily | Pleckstrin homology domain | Rho GTPase-activating Guanine Nucleotide Exchange Factors | SOS1/NGEF-like, PH domain | Spectrin/alpha-actinin |
|---|---|---|---|---|---|---|---|---|---|
|
UniProtKB:A0A8N7T881
|
1093 | ||||||||
|
UniProtKB:A0A8M9Q556
|
1088 | ||||||||
|
UniProtKB:A0A8M9Q336
|
1095 | ||||||||
|
UniProtKB:A0A8M9QBG1
|
1072 | ||||||||
|
UniProtKB:A0A8M9QK90
|
1097 |
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| Type | Name | Annotation Method | Has Havana Data | Length (nt) | Analysis |
|---|---|---|---|---|---|
| mRNA |
mcf2lb-201
(1)
|
Ensembl | 5,052 nt | ||
| mRNA |
mcf2lb-202
(1)
|
Ensembl | 1,543 nt | ||
| mRNA |
mcf2lb-203
(1)
|
Ensembl | 1,507 nt | ||
| mRNA |
mcf2lb-204
(1)
|
Ensembl | 5,025 nt | ||
| mRNA |
mcf2lb-205
(1)
|
Ensembl | 5,033 nt |
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Interactions and Pathways
No data available
Plasmids
No data available
No data available
| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | DKEY-46K9 | ZFIN Curated Data | |
| Encodes | EST | eu830 | Thisse et al., 2005 |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:XM_068218555 (1) | 4386 nt | ||
| Genomic | GenBank:BX537139 (1) | 243063 nt | ||
| Polypeptide | UniProtKB:A0A8M9QK90 (1) | 1097 aa |
- Olson, H.M., Maxfield, A., Calistri, N.L., Heiser, L.M., Qian, W., Knaut, H., Nechiporuk, A.V. (2024) RhoA GEF Mcf2lb regulates rosette integrity during collective cell migration. Development (Cambridge, England). 151(1):
- Ezhkova, D., Schwarzer, S., Spieß, S., Geffarth, M., Machate, A., Zöller, D., Stucke, J., Alexopoulou, D., Lesche, M., Dahl, A., Hans, S. (2023) Transcriptome analysis reveals an Atoh1b-dependent gene set downstream of Dlx3b/4b during early inner ear development in zebrafish. Biology Open. 12(6):
- Hoffmann, S., Schmitteckert, S., Raedecke, K., Rheinert, D., Diebold, S., Roeth, R., Weiss, B., Granzow, M., Niesler, B., Griesbeck, A., Eckstein, V., Zimmermann, W.H., Just, S., Rappold, G.A. (2021) Network-driven discovery yields new insight into Shox2-dependent cardiac rhythm control. Biochimica et biophysica acta. Gene regulatory mechanisms. 1864(4-5):194702
- Braasch, I., Gehrke, A.R., Smith, J.J., Kawasaki, K., Manousaki, T., Pasquier, J., Amores, A., Desvignes, T., Batzel, P., Catchen, J., Berlin, A.M., Campbell, M.S., Barrell, D., Martin, K.J., Mulley, J.F., Ravi, V., Lee, A.P., Nakamura, T., Chalopin, D., Fan, S., Wcisel, D., Cañestro, C., Sydes, J., Beaudry, F.E., Sun, Y., Hertel, J., Beam, M.J., Fasold, M., Ishiyama, M., Johnson, J., Kehr, S., Lara, M., Letaw, J.H., Litman, G.W., Litman, R.T., Mikami, M., Ota, T., Saha, N.R., Williams, L., Stadler, P.F., Wang, H., Taylor, J.S., Fontenot, Q., Ferrara, A., Searle, S.M., Aken, B., Yandell, M., Schneider, I., Yoder, J.A., Volff, J.N., Meyer, A., Amemiya, C.T., Venkatesh, B., Holland, P.W., Guiguen, Y., Bobe, J., Shubin, N.H., Di Palma, F., Alföldi, J., Lindblad-Toh, K., Postlethwait, J.H. (2016) The spotted gar genome illuminates vertebrate evolution and facilitates human-teleost comparisons. Nature Genetics. 48(4):427-37
- Elkon, R., Milon, B., Morrison, L., Shah, M., Vijayakumar, S., Racherla, M., Leitch, C.C., Silipino, L., Hadi, S., Weiss-Gayet, M., Barras, E., Schmid, C.D., Ait-Lounis, A., Barnes, A., Song, Y., Eisenman, D.J., Eliyahu, E., Frolenkov, G.I., Strome, S.E., Durand, B., Zaghloul, N.A., Jones, S.M., Reith, W., Hertzano, R. (2015) RFX transcription factors are essential for hearing in mice. Nature communications. 6:8549
- Mitchell, R.E., Huitema, L.F., Skinner, R.H., Brunt, L.H., Severn, C., Schulte-Merker, S., and Hammond, C.L. (2013) New tools for studying osteoarthritis genetics in zebrafish. Osteoarthritis and Cartilage. 21(2):269-278
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