Gene
tmc2b
- ID
- ZDB-GENE-060526-262
- Name
- transmembrane channel-like 2b
- Symbol
- tmc2b Nomenclature History
- Previous Names
-
- si:dkey-229d2.2
- Type
- protein_coding_gene
- Location
- Chr: 5 Mapping Details/Browsers
- Description
- Predicted to enable mechanosensitive monoatomic ion channel activity. Involved in inner ear morphogenesis and neuromast development. Acts upstream of or within detection of mechanical stimulus involved in sensory perception of sound. Predicted to be located in plasma membrane. Is expressed in inner ear; neuromast; and neuromast hair cell. Orthologous to human TMC2 (transmembrane channel like 2).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 3 figures from 2 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
- No data available
Wild Type Expression Summary
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| cwr8 | Allele with one deletion | Exon 4 | Premature Stop | TALEN | |
| cwr9 | Allele with one deletion | Exon 7 | Unknown | CRISPR | |
| ihb613 | Allele with one deletion | Unknown | Unknown | CRISPR | |
| ihb614 | Allele with one insertion | Unknown | Unknown | CRISPR | |
| sa8817 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa38469 | Allele with one point mutation | Unknown | Splice Site | ENU | |
| zf3404 | Allele with one deletion | Exon 6 | Premature Stop | CRISPR | |
| zf3405 | Allele with one deletion | Unknown | Unknown | TALEN | |
| zf3406 | Allele with one deletion | Exon 7 | Premature Stop | CRISPR |
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| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-tmc2b | (3) | |
| CRISPR2-tmc2b | (2) | |
| CRISPR3-tmc2b | Zebrafish Nomenclature Committee | |
| CRISPR4-tmc2b | Zebrafish Nomenclature Committee | |
| TALEN1-tmc2b | (2) |
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Human Disease
Domain, Family, and Site Summary
Domain Details Per Protein
| Protein | Length | TMC domain | Transmembrane channel-like protein |
|---|---|---|---|
|
UniProtKB:F1QZE9
|
892 | ||
|
UniProtKB:A0A8M6YZ60
|
955 |
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Interactions and Pathways
No data available
Plasmids
No data available
| Construct | Regulatory Region | Coding Sequence | Species | Tg Lines | Citations |
|---|---|---|---|---|---|
| Tg(myo6b:tmc2b-EGFP) |
|
| 1 | (4) |
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| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | DKEY-229D2 | ZFIN Curated Data |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_001302223 (1) | |||
| Genomic | GenBank:BX296526 (1) | 179086 nt | ||
| Polypeptide | UniProtKB:A0A8M6YZ60 (1) | 955 aa |
- Smith, E.T., Sun, P., Yu, S.K., Raible, D.W., Nicolson, T. (2023) Differential expression of mechanotransduction complex genes in auditory/vestibular hair cells in zebrafish. Frontiers in molecular neuroscience. 16:12748221274822
- Sun, P., Smith, E., Nicolson, T. (2023) Transmembrane channel-like (Tmc) subunits contribute to frequency sensitivity in the zebrafish utricle. The Journal of neuroscience : the official journal of the Society for Neuroscience. 44(1):
- Zhu, S., Chen, Z., Wang, H., McDermott, B.M. (2021) Tmc Reliance Is Biased by the Hair Cell Subtype and Position Within the Ear. Frontiers in cell and developmental biology. 8:570486
- Chen, Z., Zhu, S., Kindig, K., Wang, S., Chou, S.W., Davis, R.W., Dercoli, M.R., Weaver, H., Stepanyan, R., McDermott, B.M. (2020) Tmc proteins are essential for zebrafish hearing where Tmc1 is not obligatory. Human molecular genetics. 29(12):2004-2021
- Erickson, T., Pacentine, I.V., Venuto, A., Clemens, R., Nicolson, T. (2020) The lhfpl5 Ohnologs lhfpl5a and lhfpl5b Are Required for Mechanotransduction in Distinct Populations of Sensory Hair Cells in Zebrafish. Frontiers in molecular neuroscience. 12:320
- Kozak, E.L., Palit, S., Miranda-Rodríguez, J.R., Janjic, A., Böttcher, A., Lickert, H., Enard, W., Theis, F.J., López-Schier, H. (2020) Epithelial Planar Bipolarity Emerges from Notch-Mediated Asymmetric Inhibition of Emx2. Current biology : CB. 30(6):1142-1151.e6
- Smith, E.T., Pacentine, I., Shipman, A., Hill, M., Nicolson, T. (2020) Disruption of tmc1/2a/2b genes in zebrafish reveals subunit requirements in subtypes of inner ear hair cells. The Journal of neuroscience : the official journal of the Society for Neuroscience. 40(23):4457-4468
- Lush, M.E., Diaz, D.C., Koenecke, N., Baek, S., Boldt, H., St Peter, M.K., Gaitan-Escudero, T., Romero-Carvajal, A., Busch-Nentwich, E.M., Perera, A.G., Hall, K.E., Peak, A., Haug, J.S., Piotrowski, T. (2019) scRNA-Seq reveals distinct stem cell populations that drive hair cell regeneration after loss of Fgf and Notch signaling. eLIFE. 8:
- Pacentine, I.V., Nicolson, T. (2019) Subunits of the mechano-electrical transduction channel, Tmc1/2b, require Tmie to localize in zebrafish sensory hair cells. PLoS Genetics. 15:e1007635
- Barta, C.L., Liu, H., Chen, L., Giffen, K.P., Li, Y., Kramer, K.L., Beisel, K.W., He, D.Z. (2018) RNA-seq transcriptomic analysis of adult zebrafish inner ear hair cells. Scientific data. 5:180005
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