Figure 6—figure supplement 1.

<italic toggle='yes'>Metrns</italic> loss-of-function impairs Kupffer’s vesicle formation and function.

(A) Dorsal views of 14 hpf wild type (WT) and triplMut embryos immunostained with ZO-1. Wild type embryos display a large KV lumen with uniform ZO-1-labeled tight junction lattice. TriplMut embryos show disturbed KV lumen and dysmorphic ZO-1 lattice. (B) WT KV cells of 14 hpf Tg(sox17:GFP) show proper polarization as visualized with anti-aPKCζ immunostaining (white arrowhead). 14 hpf TriplMut embryos, in contrast, exhibit reduced polarization (white arrowhead). (C) Tracked microbeads velocity analysis in 12–14 hpf WT and triplMut embryos. The purple line marks the mean and the standard deviation (std) for each condition (WT mean = 0.96212; std = 0.048143; triplMut mean = 0.47996; std = 0.023682). Scale bars: in (A–B) 20 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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