ZFIN ID: ZDB-ETCONSTRCT-130724-3
Enhancer Trap Construct Name: Et2(E1B:GAL4FF,myl7:GFP)
Sequence Ontology ID :
Synonyms: Et(bp:Gal4FF-CR) (1), Et2(E1b:Gal4FF, myl7:GFP), Et2(E1b:GAL4FF, myl7:GFP), Et2(E1b:GAL4FF,myl7:GFP)

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Note: This enhancer trapping construct is based on pDestTol2CR3, a multisite-Gateway-based plasmid.  It contains a minimal adenovirus E1b promoter driving Gal4FF (Gal4 DNA-binding domain fused to a duplicated portion of the VP16 transcriptional activation domain; Asakawa et al., 2008) followed by the late SV40 polyA signal sequence.  Following (3’ to) the enhancer trapping construct is a transgene marker: the cmlc2 promoter driving TagRFP followed by the SV40 late polyA signal sequence; this marks the heart with red fluorescence.  The enhancer trapping construct and the transgene marker are in a tail-to-tail orientation such that they drive transcription in opposite directions.  The entire cassette (enhancer trapping construct + transgene marker) is flanked by miniTol2 transposon ends (Balciunas et al., 2006).

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CONSTRUCT COMPONENTS
Regulatory Regions: E1B
myl7 (1)
Coding Sequences: GAL4FF (1), GFP (1)
GENOMIC FEATURES THAT UTILIZE Et2(E1B:GAL4FF,myl7:GFP)
Genomic Feature Affected Genomic Regions    
zc1082aEt    
TRANSGENICS THAT UTILIZE Et2(E1B:GAL4FF,myl7:GFP)
Fish Affected Genomic Regions Phenotype Gene Expression
c264Tg; zc1082aEt 7 figures with image from Hutcheson et al., 2014
SEQUENCE INFORMATION
Type Accession # Length (bp/aa) Analysis
RNA GenBank:KC800699
OTHER Et2(E1B:GAL4FF,myl7:GFP) ENHANCER TRAP CONSTRUCT PAGESNo links to external sites
CITATIONS (5)