ZFIN ID: ZDB-ETCONSTRCT-130724-2
Enhancer Trap Construct Name: Et1(E1B:GAL4FF,myl7:GFP)
Sequence Ontology ID :
Synonyms: Et(bp:Gal4FF-CG) (1), Et1(E1b:Gal4FF, myl7:GFP), Et1(E1b:GAL4FF, myl7:GFP), Et1(E1b:GAL4FF,myl7:GFP)

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Note: This enhancer trapping construct is based on pDestTol2CG3, a multisite-Gateway-based plasmid.  It contains a minimal adenovirus E1b promoter driving Gal4FF (Gal4 DNA-binding domain fused to a duplicated portion of the VP16 transcriptional activation domain; Asakawa et al., 2008) followed by the late SV40 polyA signal sequence.  Following (3’ to) the enhancer trapping construct is a transgene marker: the cmlc2 promoter driving EGFP followed by the SV40 late polyA signal sequence; this marks the heart with green fluorescence.  The enhancer trapping construct and the transgene marker are in a tail-to-tail orientation such that they drive transcription in opposite directions.  The entire cassette (enhancer trapping construct + transgene marker) is flanked by miniTol2 transposon ends (Balciunas et al., 2006).

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CONSTRUCT COMPONENTS
Regulatory Regions: E1B
myl7 (1)
Coding Sequences: GAL4FF (1), GFP (1)
GENOMIC FEATURES THAT UTILIZE Et1(E1B:GAL4FF,myl7:GFP)
Genomic Feature Affected Genomic Regions    
zc1080aEt    
zc1081aEt    
zc1083aEt    
zc1084aEt    
zc1087aEt    
zc1088aEt    
zc1090aEt    
zc1091aEt    
zc1093aEt    
TRANSGENICS THAT UTILIZE Et1(E1B:GAL4FF,myl7:GFP)
Fish Affected Genomic Regions Phenotype Gene Expression
c264Tg; zc1080aEt(AB) 4 figures with image from Hutcheson et al., 2014
c264Tg; zc1081aEt 6 figures with image from Hutcheson et al., 2014
c264Tg; zc1083aEt 7 figures with image from Hutcheson et al., 2014
c264Tg; zc1084aEt 9 figures with image from Hutcheson et al., 2014
c264Tg; zc1087aEt(AB) 6 figures with image from Hutcheson et al., 2014
c264Tg; zc1088aEt 9 figures with image from Hutcheson et al., 2014
c264Tg; zc1090aEt(AB) 9 figures with image from Hutcheson et al., 2014
c264Tg; zc1091aEt 3 figures with image from Hutcheson et al., 2014
c264Tg; zc1093aEt(AB)
s1999tTg; zc1087aEt
s1999tTg; zc1090aEt
s1999tTg; zc1093aEt
zc1080aEt
SEQUENCE INFORMATION
Type Accession # Length (bp/aa) Analysis
RNA GenBank:KC800698
OTHER Et1(E1B:GAL4FF,myl7:GFP) ENHANCER TRAP CONSTRUCT PAGESNo links to external sites
CITATIONS (5)