IMAGE:7152122

ID

ZDB-EST-080917-14

Name

IMAGE:7152122

Symbol

IMAGE:7152122

Previous Names

None

Type

EST

Location

Unmapped

Genome Resources

None

Species

Danio rerio

Library

GISZF001_ra

Digest

Insert Size

2800

Cloning Site

Sfi A - Sfi B

Vector Type

plasmid

Vector

pDNR-LIB

Polymerase

T3 RNA polymerase

PCR Amplification

Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TGG ATA ACC GTA TTA CCG CC 3'
5' CGC GCA ATT AAC CCT CAC TAA AGC ACT AGT CAT ACC AGG ATC 3'

Suppliers

None

Quality

( Probe is difficult to use. Generally basal level of expression with more intense labeling in particular structure. )

Protocol

Thisse in situ hybridization protocol

Note

None