EST

IMAGE:7159784

ID
ZDB-EST-080917-113
Name
IMAGE:7159784
Symbol
IMAGE:7159784
Previous Names
None
Type
EST
Location
Unmapped
Genome Resources
None
Species
Danio rerio
Library
GISZF001_ra
Digest
Insert Size
2000
Cloning Site
Sfi A - Sfi B
Vector Type
plasmid
Vector
pDNR-LIB
Polymerase
T3 RNA polymerase
PCR Amplification
Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TGG ATA ACC GTA TTA CCG CC 3'
5' CGC GCA ATT AAC CCT CAC TAA AGC ACT AGT CAT ACC AGG ATC 3'
Quality
( Simple to use, intense expression pattern restricted to a few structures )
Protocol
Thisse in situ hybridization protocol
Note
None
Gene Expression
Directly Submitted Expression Data
6 figures (20 images) from Thisse et al., 2004 [IMAGE:7159784]
Marker Relationships
Sequences
Citations