IMAGE:7162625

ID

ZDB-EST-060810-440

Name

IMAGE:7162625

Symbol

IMAGE:7162625

Previous Names

• FR_6441 (1)

Type

EST

Location

Unmapped

Genome Resources

None

Species

Danio rerio

Library

GISZF001_ra

Digest

Insert Size

1200

Cloning Site

Sfi A - Sfi B

Vector Type

plasmid

Vector

pDNR-LIB

Polymerase

T3 RNA polymerase

PCR Amplification

Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TGG ATA ACC GTA TTA CCG CC 3'
5' CGC GCA ATT AAC CCT CAC TAA AGC ACT AGT CAT ACC AGG ATC 3'

Suppliers

None

Protocol

Thisse in situ hybridization protocol

Note

None