EST
IMAGE:7151115
- ID
- ZDB-EST-050309-180
- Name
- IMAGE:7151115
- Symbol
- IMAGE:7151115
- Previous Names
-
- FR3003 (1)
- Type
- EST
- Location
- Unmapped
- Genome Resources
- None
- Species
- Danio rerio
- Library
- GISZF001_ra
- Digest
- Insert Size
- 1100
- Cloning Site
- Sfi A - Sfi B
- Vector Type
- plasmid
- Vector
- pDNR-LIB
- Polymerase
- T3 RNA polymerase
- PCR Amplification
-
Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TGG ATA ACC GTA TTA CCG CC 3'
5' CGC GCA ATT AAC CCT CAC TAA AGC ACT AGT CAT ACC AGG ATC 3' - Suppliers
- None
- Protocol
- Thisse in situ hybridization protocol
- Note
- None
Gene Expression
- Directly Submitted Expression Data
- 1 figure (1 image) from Thisse et al., 2004 [IMAGE:7151115]
Marker Relationships
Sequences
Citations