IMAGE:5611934

ID

ZDB-EST-040908-1

Name

IMAGE:5611934

Symbol

IMAGE:5611934

Previous Names

• FR581 (1)

Type

EST

Location

Unmapped

Genome Resources

None

Species

Danio rerio

Library

Sugano SJD adult male

Digest

Insert Size

1942

Cloning Site

EcoRI-PstI

Vector Type

Plasmid

Vector

pME18S-FL3

Polymerase

T3 RNA polymerase

PCR Amplification

Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TGTACGGAAGTGTTACTTCTGCTC3'
5'GGATCCATTAACCCTCACTAAAGGGAAGGCCGCGACCTGCAGCTC3'

Suppliers

None

Protocol

Thisse in situ hybridization protocol

Note

Danio rerio cDNA clone MGC:66189 IMAGE:5611934, complete cds