EST
cb713
- ID
- ZDB-EST-030521-31
- Name
- cb713
- Symbol
- cb713
- Previous Names
- None
- Type
- EST
- Location
- Unmapped
- Genome Resources
- None
- Species
- Danio rerio
- Library
- oligo-dT primed gastrula stage
- Digest
- PCR amplification
- Insert Size
- 2000
- Cloning Site
- Sal I - Not I
- Vector Type
- Plasmid
- Vector
- pZIPLOX
- Polymerase
- T3 RNA polymerase
- PCR Amplification
-
(replaces the SP6 promoter with a T3 promoter)
T3p : 5' GGA TCC ATT AAC CCT CAC TAA AGG GAA GAG CTA TGA CGT CGC AT 3'
T7p : 5' TAA TAC GAC TCA CTA TAG GG 3' - Suppliers
- Quality
- ( Nice strong expression pattern )
- Protocol
- Thisse in situ hybridization protocol
- Note
- None
Gene Expression
- Directly Submitted Expression Data
- 6 figures (21 images) from Thisse et al., 2001 [cb713]
Marker Relationships
Sequences
Citations