cb489

ID

ZDB-EST-030429-36

Name

cb489

Symbol

cb489

Previous Names

None

Type

EST

Location

Unmapped

Genome Resources

None

Species

Danio rerio

Library

oligo-dT primed 26-somite stage

Digest

PCR amplification

Insert Size

4500

Cloning Site

EcoRI-SalI

Vector Type

Plasmid

Vector

pSPORT1

Polymerase

T3 RNA polymerase

PCR Amplification

(replaces the SP6 promoter with a T3 promoter)
T3p : 5' GGA TCC ATT AAC CCT CAC TAA AGG GAA GAG CTA TGA CGT CGC AT 3'
T7p : 5' TAA TAC GAC TCA CTA TAG GG 3'

Suppliers

None

Quality

( Nice strong expression pattern )

Protocol

Thisse in situ hybridization protocol

Note

None