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ZIRC
ZFIN ID: ZDB-EST-021126-22
EST Name: cb511
Sequence Ontology ID : SO:0000345
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(Including Attributions)
Species: Danio rerio
Library: oligo-dT primed segmentation stage
Digest: NotI
Insert Size: 1200
Cloning Site: EcoRI-SalI Digest
Vector Type: Plasmid
Vector: pSPORT1
Polymerase: T3 RNA polymerase
PCR Amplification: (replaces the SP6 promoter with a T3 promoter) T3p : 5' GGA TCC ATT AAC CCT CAC TAA AGG GAA GAG CTA TGA CGT CGC AT 3' T7p : 5' TAA TAC GAC TCA CTA TAG GG 3'
Source:
Quality: Rating 4 ( Nice strong expression pattern )
Protocol: Thisse in situ hybridization protocol
Location: Unmapped
Note: The probe available from ZIRC is a 1200 nt subclone of AF371368 in the pSport vector and should be amplified using PCR.
GBROWSE
GENE EXPRESSION
Directly Submitted Expression Data: 7 figures (49 images) from Thisse et al., 2001 [cb511]
MARKER RELATIONSHIPS
cb511 Is Encoded by: [Gene] spry4 (2)
SEQUENCE INFORMATION No data available
OTHER cb511 EST PAGES No links to external sites
CITATIONS (2)