EST
cb160
- ID
- ZDB-EST-020409-4
- Name
- cb160
- Symbol
- cb160
- Previous Names
- None
- Type
- EST
- Location
- Unmapped
- Genome Resources
- None
- Species
- Danio rerio
- Library
- oligo-dT primed segmentation stage
- Digest
- NotI
- Insert Size
- 2300
- Cloning Site
- EcoRI-XhoI
- Vector Type
- Plasmid
- Vector
- pBS-IISK+
- Polymerase
- T7 RNA polymerase
- PCR Amplification
- Suppliers
- Quality
- ( Simple to use, intense expression pattern restricted to a few structures )
- Protocol
- Thisse in situ hybridization protocol
- Note
-
The following is from Vogel AM and Gerster T. Mech Dev 1999 Jul;85(1-2):133-46
bik elements are transcribed in all early mesendodermal cells. This expression pattern is generated by a promoter located in the U3 region of the LTR. We show that bik is activated through the activin/Vg1 signaling pathway in an immediate early fashion. This activation critically depends on a sequence motif that occurs among others also in the Xenopus Mix2 activin response element (ARE). It has been shown that the Mix2 ARE binds FAST- 1, which complexes with Smad proteins to form a multi-protein complex. We confirm that also the bik ARE can be bound by FAST-1 in vitro. In animal cap experiments we demonstrate that this binding site is required for activin-induced transcriptional activation mediated by FAST and Smad-type proteins.
Gene Expression
- Directly Submitted Expression Data
- 6 figures (8 images) from Thisse et al., 2001 [cb160]
Marker Relationships
Sequences
Citations