GAVPO

ID

ZDB-EFG-210513-1

Name

GAVPO

Symbol

GAVPO

Previous Names

• GAVPO (1)

Type

engineered_foreign_gene

Location

Unmapped

Note

The GAVPO transactivator contains a Gal4 DNA-binding domain, a variant of the VIVID light-oxygen-voltage (LOV) domain, and the p65 transcriptional activation domain. Upon exposure to blue light, the LOV domain undergoes a photo-reductive reaction that forms a covalent adduct between its flavin adenine dinucleotide chromophore and a neighboring cysteine residue. The adduct stabilizes a conformational state that promotes LOV domain self-association, generating a transcriptionally active GAVPO dimer. Light-activated GAVPO then drives the expression of upstream activating sequence (UAS)-controlled genes until it reverts to the inactive monomer with a half-life of∼2h (Wang et al. 2012).