CRISPR

CRISPR3-dpy19l1l

ID

ZDB-CRISPR-260511-1

Name

CRISPR3-dpy19l1l

Previous Names

None

Target

dpy19l1l (1)

Sequence

5' - AGGAGAGGGTCTGGATCCAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
nkz5	dpy19l1l

Expression

Gene expression in Wild Types + CRISPR3-dpy19l1l

No data available

Phenotype

Phenotype resulting from CRISPR3-dpy19l1l

Phenotype	Fish	Figures
trunk bent, abnormal	AB/TU + CRISPR3-dpy19l1l	Fig. 2 from Usami et al., 2026
trunk curved, abnormal	AB/TU + CRISPR3-dpy19l1l	Fig. 2 from Usami et al., 2026

Phenotype of all Fish created by or utilizing CRISPR3-dpy19l1l

Phenotype	Fish	Conditions	Figures
post-vent region curved, abnormal	dpy19l1l^nkz5/nkz5 (AB/TU)	standard conditions	Fig. 2 from Usami et al., 2026
post-vent region increased curvature, abnormal	dpy19l1l^nkz5/nkz5 (AB/TU)	standard conditions	Fig. 2 from Usami et al., 2026
trunk bent, abnormal	dpy19l1l^nkz5/nkz5 (AB/TU)	standard conditions	Fig. 2 from Usami et al., 2026
post-vent region curved, abnormal	dpy19l1l^nkz5/nkz5 (AB/TU)	standard conditions	Fig. 2 from Usami et al., 2026
trunk rotational curvature, abnormal	dpy19l1l^nkz5/nkz5 (AB/TU)	standard conditions	Fig. 2 from Usami et al., 2026
trunk curved, abnormal	dpy19l1l^nkz5/nkz5 (AB/TU)	standard conditions	Fig. 2 from Usami et al., 2026
trunk curved, abnormal	dpy19l1l^nkz5/nkz5 (AB/TU)	standard conditions	Fig. 2 from Usami et al., 2026
trunk bent, abnormal	dpy19l1l^nkz5/nkz5 (AB/TU)	standard conditions	Fig. 2 from Usami et al., 2026
trunk bent, abnormal	AB/TU + CRISPR3-dpy19l1l	control	Fig. 2 from Usami et al., 2026
trunk curved, abnormal	AB/TU + CRISPR3-dpy19l1l	control	Fig. 2 from Usami et al., 2026
Reissner's fiber EGFP expression absent, abnormal	dpy19l1l^nkz5/nkz5; sspo^ut24Tg/+ (AB/TU)	standard conditions	Fig. 4 from Usami et al., 2026
post-vent region increased curvature, abnormal	dpy19l1l^nkz5/nkz5; sspo^ut24Tg/+ (AB/TU)	standard conditions	Fig. 4 from Usami et al., 2026
Reissner's fiber absence of anatomical entity, abnormal	dpy19l1l^nkz5/nkz5; sspo^ut24Tg/+ (AB/TU)	standard conditions	Fig. 4 from Usami et al., 2026
floor plate basal side EGFP expression spatial pattern, abnormal	dpy19l1l^nkz5/nkz5; sspo^ut24Tg/+ (AB/TU)	standard conditions	Fig. 4 from Usami et al., 2026
post-vent region curved, abnormal	dpy19l1l^nkz5/nkz5; sspo^ut24Tg/+ (AB/TU)	standard conditions	Fig. 4 from Usami et al., 2026
floor plate basal side EGFP expression mislocalised, abnormal	dpy19l1l^nkz5/nkz5; sspo^ut24Tg/+ (AB/TU)	standard conditions	Fig. 4 from Usami et al., 2026

Citations