CRISPR

CRISPR1-gtf3aa

ID

ZDB-CRISPR-260421-10

Name

CRISPR1-gtf3aa

Previous Names

None

Target

gtf3aa (1)

Sequence

5' - ACGCGTATTACAACCGAGAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ihb950	gtf3aa

Expression

Gene expression in Wild Types + CRISPR1-gtf3aa

No data available

Phenotype

Phenotype resulting from CRISPR1-gtf3aa

No data available

Phenotype of all Fish created by or utilizing CRISPR1-gtf3aa

Phenotype	Fish	Conditions	Figures
liver gc expression decreased amount, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 10 from Luo et al., 2025
whole organism large ribosomal subunit decreased amount, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 5 from Luo et al., 2025
whole organism fads2 expression decreased amount, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 9 from Luo et al., 2025
whole organism rpl35 expression decreased amount, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 5 from Luo et al., 2025
whole organism cpt1b expression decreased amount, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 9 from Luo et al., 2025
heart increased size, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 6 , Fig. 7 from Luo et al., 2025
whole organism dead, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 4 from Luo et al., 2025
whole organism fabp6 expression decreased amount, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 9 from Luo et al., 2025
whole organism decreased length, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 6 , Fig. 7 from Luo et al., 2025
whole organism scp2a expression decreased amount, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 9 from Luo et al., 2025
whole organism ribosome increased amount, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 5 from Luo et al., 2025
liver uox expression decreased amount, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 10 from Luo et al., 2025
whole organism pdpk1a expression decreased amount, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 9 from Luo et al., 2025
liver fabp10a expression decreased amount, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 8 , Fig. 10 from Luo et al., 2025
whole organism hmgcs1 expression decreased amount, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 9 from Luo et al., 2025
whole organism cpt2 expression decreased amount, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 9 from Luo et al., 2025
whole organism viability, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 4 from Luo et al., 2025
yolk syncytial layer increased size, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 6 , Fig. 7 from Luo et al., 2025
intestine fabp2 expression decreased amount, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 8 from Luo et al., 2025
eye decreased size, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 6 , Fig. 7 from Luo et al., 2025
whole organism acadl expression decreased amount, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 9 from Luo et al., 2025
whole organism cd36 expression decreased amount, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 9 from Luo et al., 2025
liver tfa expression decreased amount, abnormal	gtf3aa^{ihb950/ihb950} (AB)	standard conditions	Fig. 8 , Fig. 10 from Luo et al., 2025

Citations