CRISPR

CRISPR1-pax4

ID
ZDB-CRISPR-260220-3
Name
CRISPR1-pax4
Previous Names
None
Target
Sequence
5' - GATGATTGAGCTGGCGACTG - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "AGG" at the 3' end,
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
cq154 pax4
Expression
Gene expression in Wild Types + CRISPR1-pax4
No data available
Phenotype
Phenotype resulting from CRISPR1-pax4
No data available
Phenotype of all Fish created by or utilizing CRISPR1-pax4
Phenotype Fish Conditions Figures
pancreatic epsilon cell ghrl expression increased amount, abnormal ghrlzf4264Tg/zf4264Tg; pax4cq154/cq154 standard conditions Fig. 4. with image from Yu et al., 2023
pancreatic epsilon cell ghrl expression increased amount, abnormal ghrlzf4264Tg/zf4264Tg; pax4cq154/cq154 chemical treatment by environment: metronidazole Fig. 4. with image from Yu et al., 2023
pancreatic epsilon cell CFP expression amount, ameliorated pax4cq154/cq154; cq151Tg/cq151Tg; cq67Tg/cq67Tg chemical treatment by environment: metronidazole Fig. 3. with image from Yu et al., 2023
pancreatic epsilon cell pancreas regeneration increased process quality, abnormal pax4cq154/cq154; cq151Tg/cq151Tg; cq67Tg/cq67Tg chemical treatment by environment: metronidazole Fig. 3. with image from Yu et al., 2023
pancreatic epsilon cell Dendra2 expression amount, ameliorated pax4cq154/cq154; cq151Tg/cq151Tg; cq67Tg/cq67Tg chemical treatment by environment: metronidazole Fig. 3. with image from Yu et al., 2023
pancreatic epsilon cell transdifferentiation increased occurrence, abnormal pax4cq154/cq154; cq151Tg/cq151Tg; cq67Tg/cq67Tg chemical treatment by environment: metronidazole Fig. 3. with image from Yu et al., 2023
pancreatic epsilon cell pancreas regeneration increased process quality, exacerbated pax4cq154/cq154; cq156Tg/cq156Tg; cq67Tg/cq67Tg; zf4263Tg/zf4263Tg chemical treatment by environment: afimoxifene, chemical treatment by environment: metronidazole Fig. 3. with image from Yu et al., 2023
pancreatic epsilon cell transdifferentiation increased occurrence, exacerbated pax4cq154/cq154; cq156Tg/cq156Tg; cq67Tg/cq67Tg; zf4263Tg/zf4263Tg chemical treatment by environment: afimoxifene, chemical treatment by environment: metronidazole Fig. 3. with image from Yu et al., 2023
Citations
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