CRISPR

CRISPR3-hoxb5a

ID

ZDB-CRISPR-250123-5

Name

CRISPR3-hoxb5a

Previous Names

None

Target

hoxb5a (1)

Sequence

5' - TATTTGGGGAGCTTGGCCAT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
sud125	hoxb5a

Expression

Gene expression in Wild Types + CRISPR3-hoxb5a

No data available

Phenotype

Phenotype resulting from CRISPR3-hoxb5a

No data available

Phenotype of all Fish created by or utilizing CRISPR3-hoxb5a

Phenotype	Fish	Conditions	Figures
vertebra 2 decreased size, abnormal	hoxb5a^{sud125/sud125}	standard conditions	Fig. 3 from Maeno et al., 2024
parapophysis 2 shortened, abnormal	hoxb5a^{sud125/sud125}	standard conditions	Fig. 4 from Maeno et al., 2024
parapophysis 2 shortened, abnormal	hoxb4a^{sud131/sud131}; hoxb5a^{sud125/sud125}	standard conditions	Fig. 4 from Maeno et al., 2024
scaphium absent, abnormal	hoxb4a^{sud131/sud131}; hoxb5a^{sud125/sud125}	standard conditions	Fig. 4 from Maeno et al., 2024
scaphium absent, abnormal	hoxb4a^{sud144/sud144}; hoxb5a^{sud125/sud125}	standard conditions	Fig. 4 from Maeno et al., 2024
parapophysis 2 shortened, abnormal	hoxb4a^{sud144/sud144}; hoxb5a^{sud125/sud125}	standard conditions	Fig. 4 from Maeno et al., 2024
vertebra regionalization decreased process quality, abnormal	hoxb4a^{sud144/sud144}; hoxb5a^{sud125/sud125}	standard conditions	Fig. 4 from Maeno et al., 2024
vertebra 3 fused with vertebra 4, abnormal	hoxb5a^{sud125/sud125}; hoxb5b^{sud136/sud136}	standard conditions	Fig. 3 from Maeno et al., 2024
vertebra 3 malformed, abnormal	hoxb5a^{sud125/sud125}; hoxb5b^{sud136/sud136}	standard conditions	Fig. 3 from Maeno et al., 2024
vertebra 2 decreased size, exacerbated	hoxb5a^{sud125/sud125}; hoxb5b^{sud136/sud136}	standard conditions	Fig. 3 from Maeno et al., 2024
os suspensorium absent, abnormal	hoxb5a^{sud125/sud125}; hoxb5b^{sud136/sud136}	standard conditions	Fig. 3 from Maeno et al., 2024
vertebra regionalization decreased process quality, abnormal	hoxb5a^{sud125/sud125}; hoxb5b^{sud136/sud136}	standard conditions	Fig. 3 from Maeno et al., 2024
vertebra 4 parapophysis decreased size, abnormal	hoxb5a^{sud125/sud125}; hoxb5b^{sud136/sud136}	standard conditions	Fig. 3 from Maeno et al., 2024

Citations