CRISPR

CRISPR1-pierce2

ID
ZDB-CRISPR-240311-1
Name
CRISPR1-pierce2
Previous Names
None
Target
Sequence
5' - GGGTCGAGCGCCGTAATCAC - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "TGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
sq5721 pierce2
Expression
Gene expression in Wild Types + CRISPR1-pierce2
No data available
Phenotype
Phenotype resulting from CRISPR1-pierce2
No data available
Phenotype of all Fish created by or utilizing CRISPR1-pierce2
Phenotype Fish Conditions Figures
heart looping decreased process quality, abnormal pierce1sq5720/sq5720; pierce2sq5721/sq5721 (AB) standard conditions Figure 6. with image from Gui et al., 2021
heart tube ab-a4.1025 labeling spatial pattern, abnormal pierce1sq5720/sq5720; pierce2sq5721/sq5721 (AB) standard conditions Figure 6. with image from Gui et al., 2021
lateral plate mesoderm spaw expression spatial pattern, abnormal pierce1sq5720/sq5720; pierce2sq5721/sq5721 (AB) standard conditions Figure 6. with image from Gui et al., 2021
embryonic heart tube left/right pattern formation decreased process quality, abnormal pierce1sq5720/sq5720; pierce2sq5721/sq5721 (AB) standard conditions Figure 6. with image from Gui et al., 2021
post-vent region curved ventral, abnormal pierce1sq5720/sq5720; pierce2sq5721/sq5721 (AB) standard conditions Figure 6. with image from Gui et al., 2021
lateral plate mesoderm lft2 expression spatial pattern, abnormal pierce1sq5720/sq5720; pierce2sq5721/sq5721 (AB) standard conditions Figure 6. with image from Gui et al., 2021
Kupffer's vesicle outer dynein arm absence of anatomical entity, abnormal pierce1sq5720/sq5720; pierce2sq5721/sq5721 (AB) standard conditions Figure 6. with image from Gui et al., 2021
heart jogging decreased process quality, abnormal pierce1sq5720/sq5720; pierce2sq5721/sq5721 (AB) standard conditions Figure 6. with image from Gui et al., 2021
Citations
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.