CRISPR

CRISPR2-pkd1a

ID

ZDB-CRISPR-240304-1

Name

CRISPR2-pkd1a

Previous Names

None

Target

pkd1a (1)

Sequence

5' - GGTGCGGCGTCTGCGGCCTGAGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
bns507	pkd1a

Expression

Gene expression in Wild Types + CRISPR2-pkd1a

No data available

Phenotype

Phenotype resulting from CRISPR2-pkd1a

No data available

Phenotype of all Fish created by or utilizing CRISPR2-pkd1a

Phenotype	Fish	Conditions	Figures
atrioventricular valve morphology, abnormal	pkd1a^{bns507/bns507}	standard conditions	Fig. 1 from Juan et al., 2023
atrioventricular valve development disrupted, abnormal	pkd1a^{bns507/bns507}	standard conditions	Fig. 1 from Juan et al., 2023
pericardium edematous, abnormal	pkd1a^{bns507/bns507}	standard conditions	Fig. 1 from Juan et al., 2023
heart blood circulation disrupted, abnormal	pkd1a^{bns507/bns507}	standard conditions	Fig. 2 from Juan et al., 2023
blood circulation disrupted, abnormal	pkd1a^{bns507/bns507}	standard conditions	Fig. 1 from Juan et al., 2023
heart blood circulation disrupted, abnormal	pkd1a^{bns507/bns507}; pkd1l1^{bns394/bns394}	standard conditions	Fig. 2 from Juan et al., 2023
heart blood circulation disrupted, abnormal	pkd2^tc321/tc321; pkd1a^{bns507/bns507}	standard conditions	Fig. 2 from Juan et al., 2023
heart klf2b expression increased amount, abnormal	pkd2^tc321/tc321; pkd1a^{bns507/bns507}	standard conditions	Fig. 5 from Juan et al., 2023
heart klf2a expression increased amount, abnormal	pkd2^tc321/tc321; pkd1a^{bns507/bns507}	standard conditions	Fig. 5 from Juan et al., 2023
heart klf2a expression increased amount, abnormal	pkd2^tc321/tc321; pkd1a^{bns507/bns507}; pkd1l1^{bns394/bns394}	standard conditions	Fig. 5 from Juan et al., 2023
blood circulation disrupted, abnormal	pkd2^tc321/tc321; pkd1a^{bns507/bns507}; pkd1l1^{bns394/bns394}	standard conditions	Fig. 6 from Juan et al., 2023
atrioventricular canal endocardium klf2a expression increased amount, abnormal	pkd2^tc321/tc321; pkd1a^{bns507/bns507}; pkd1l1^{bns394/bns394}	standard conditions	Fig. 5 from Juan et al., 2023
heart blood circulation disrupted, exacerbated	pkd2^tc321/tc321; pkd1a^{bns507/bns507}; pkd1l1^{bns394/bns394}	standard conditions	Fig. 2 from Juan et al., 2023
heart klf2b expression increased amount, abnormal	pkd2^tc321/tc321; pkd1a^{bns507/bns507}; pkd1l1^{bns394/bns394}	standard conditions	Fig. 5 from Juan et al., 2023
atrioventricular valve development disrupted, abnormal	pkd2^tc321/tc321; pkd1a^{bns507/bns507}; pkd1l1^{bns394/bns394}	standard conditions	Fig. 2 from Juan et al., 2023
atrioventricular canal endocardium klf2b expression increased amount, abnormal	pkd2^tc321/tc321; pkd1a^{bns507/bns507}; pkd1l1^{bns394/bns394}	standard conditions	Fig. 5 from Juan et al., 2023
atrioventricular canal calcium ion decreased amount, abnormal	pkd2^tc321/tc321; pkd1a^{bns507/bns507}; pkd1l1^{bns394/bns394}; nkUAShspzGCaMP6s13aTg; ubs4Tg	standard conditions	Fig. 4 from Juan et al., 2023

Citations