CRISPR

CRISPR4-urp1

ID

ZDB-CRISPR-240124-1

Name

CRISPR4-urp1

Previous Names

None

Target

Sequence

5' - GCAGCAAAATGGATTTGTATG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
mhn3	urp1

Expression

Gene expression in Wild Types + CRISPR4-urp1

No data available

Phenotype

Phenotype resulting from CRISPR4-urp1

No data available

Phenotype of all Fish created by or utilizing CRISPR4-urp1

Phenotype	Fish	Conditions	Figures
male organism vertebral column bent, abnormal	urp1^mhn3/+; urp2^mhn4/mhn4	standard conditions	Fig. 3 from Gaillard et al., 2023
female organism vertebral column bent, abnormal	urp1^mhn3/+; urp2^mhn4/mhn4	standard conditions	Fig. 3 from Gaillard et al., 2023
whole organism irg1l expression increased amount, abnormal	urp1^mhn3/mhn3; urp2^mhn4/mhn4	standard conditions	Fig. 5 from Gaillard et al., 2023
female organism vertebral column bent, exacerbated	urp1^mhn3/mhn3; urp2^mhn4/mhn4	standard conditions	Fig. 2, Fig. 3 from Gaillard et al., 2023
whole organism il1b expression increased amount, abnormal	urp1^mhn3/mhn3; urp2^mhn4/mhn4	standard conditions	Fig. 5 from Gaillard et al., 2023
vertebral column bent, ameliorated	urp1^mhn3/mhn3; urp2^mhn4/mhn4	chemical treatment by environment: N-acetyl-L-cysteine	Fig. 5 from Gaillard et al., 2023
whole organism il11a expression increased amount, abnormal	urp1^mhn3/mhn3; urp2^mhn4/mhn4	standard conditions	Fig. 5 from Gaillard et al., 2023
vertebral column bent, abnormal	urp1^mhn3/mhn3; urp2^mhn4/mhn4	standard conditions	Fig. 2, Fig. 5 from Gaillard et al., 2023
male organism vertebral column bent, exacerbated	urp1^mhn3/mhn3; urp2^mhn4/mhn4	standard conditions	Fig. 2, Fig. 3 from Gaillard et al., 2023

Citations