CRISPR

CRISPR7-kars1

ID

ZDB-CRISPR-230602-5

Name

CRISPR7-kars1

Previous Names

None

Target

kars1 (1)

Sequence

5' - GGCTTTGCTACTGCGCCACC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Genome Build: GRCz11Chromosome: 7

Constructs

No data available

Genomic Features

No data available

Expression

Gene expression in Wild Types + CRISPR7-kars1

No data available

Phenotype

Phenotype resulting from CRISPR7-kars1

No data available

Phenotype of all Fish created by or utilizing CRISPR7-kars1

Phenotype	Fish	Conditions	Figures
brain spongy, abnormal	NHGRI-1 + CRISPR3-kars1 + CRISPR4-kars1 + CRISPR5-kars1 + CRISPR6-kars1 + CRISPR7-kars1	standard conditions	Fig. 4 from Lin et al., 2021
retina layer formation disrupted, abnormal	NHGRI-1 + CRISPR3-kars1 + CRISPR4-kars1 + CRISPR5-kars1 + CRISPR6-kars1 + CRISPR7-kars1	standard conditions	Fig. 4 from Lin et al., 2021
head decreased size, abnormal	NHGRI-1 + CRISPR3-kars1 + CRISPR4-kars1 + CRISPR5-kars1 + CRISPR6-kars1 + CRISPR7-kars1	standard conditions	Fig. 4 from Lin et al., 2021
brain vacuolated, abnormal	NHGRI-1 + CRISPR3-kars1 + CRISPR4-kars1 + CRISPR5-kars1 + CRISPR6-kars1 + CRISPR7-kars1	standard conditions	Fig. 4 from Lin et al., 2021
eye decreased size, abnormal	NHGRI-1 + CRISPR3-kars1 + CRISPR4-kars1 + CRISPR5-kars1 + CRISPR6-kars1 + CRISPR7-kars1	standard conditions	Fig. 4 from Lin et al., 2021
whole organism viability, abnormal	NHGRI-1 + CRISPR3-kars1 + CRISPR4-kars1 + CRISPR5-kars1 + CRISPR6-kars1 + CRISPR7-kars1	standard conditions	Fig. 4 from Lin et al., 2021
eye decreased volume, abnormal	NHGRI-1 + CRISPR3-kars1 + CRISPR4-kars1 + CRISPR5-kars1 + CRISPR6-kars1 + CRISPR7-kars1	standard conditions	Fig. 4 from Lin et al., 2021
retina morphology, abnormal	NHGRI-1 + CRISPR3-kars1 + CRISPR4-kars1 + CRISPR5-kars1 + CRISPR6-kars1 + CRISPR7-kars1	standard conditions	Fig. 4 from Lin et al., 2021
head size, ameliorated	NHGRI-1 + CRISPR19-tp53 + CRISPR20-tp53 + CRISPR21-tp53 + CRISPR3-kars1 + CRISPR4-kars1 + CRISPR5-kars1 + CRISPR6-kars1 + CRISPR7-kars1	standard conditions	Fig. 4 from Lin et al., 2021
whole organism viability, ameliorated	NHGRI-1 + CRISPR19-tp53 + CRISPR20-tp53 + CRISPR21-tp53 + CRISPR3-kars1 + CRISPR4-kars1 + CRISPR5-kars1 + CRISPR6-kars1 + CRISPR7-kars1	standard conditions	Fig. 4 from Lin et al., 2021
retina morphology, ameliorated	NHGRI-1 + CRISPR19-tp53 + CRISPR20-tp53 + CRISPR21-tp53 + CRISPR3-kars1 + CRISPR4-kars1 + CRISPR5-kars1 + CRISPR6-kars1 + CRISPR7-kars1	standard conditions	Fig. 4 from Lin et al., 2021
eye size, ameliorated	NHGRI-1 + CRISPR19-tp53 + CRISPR20-tp53 + CRISPR21-tp53 + CRISPR3-kars1 + CRISPR4-kars1 + CRISPR5-kars1 + CRISPR6-kars1 + CRISPR7-kars1	standard conditions	Fig. 4 from Lin et al., 2021
brain structure, ameliorated	NHGRI-1 + CRISPR19-tp53 + CRISPR20-tp53 + CRISPR21-tp53 + CRISPR3-kars1 + CRISPR4-kars1 + CRISPR5-kars1 + CRISPR6-kars1 + CRISPR7-kars1	standard conditions	Fig. 4 from Lin et al., 2021

Citations

Include unpublished citations

Lin, S.J., Vona, B., Barbalho, P.G., Kaiyrzhanov, R., Maroofian, R., Petree, C., Severino, M., Stanley, V., Varshney, P., Bahena, P., Alzahrani, F., Alhashem, A., Pagnamenta, A.T., Aubertin, G., Estrada-Veras, J.I., Hernández, H.A.D., Mazaheri, N., Oza, A., Thies, J., Renaud, D.L., Dugad, S., McEvoy, J., Sultan, T., Pais, L.S., Tabarki, B., Villalobos-Ramirez, D., Rad, A., Genomics England Research Consortium, Galehdari, H., Ashrafzadeh, F., Sahebzamani, A., Saeidi, K., Torti, E., Elloumi, H.Z., Mora, S., Palculict, T.B., Yang, H., Wren, J.D., Ben Fowler, ., Joshi, M., Behra, M., Burgess, S.M., Nath, S.K., Hanna, M.G., Kenna, M., Merritt, J.L., Houlden, H., Karimiani, E.G., Zaki, M.S., Haaf, T., Alkuraya, F.S., Gleeson, J.G., Varshney, G.K. (2021) Biallelic variants in KARS1 are associated with neurodevelopmental disorders and hearing loss recapitulated by the knockout zebrafish. Genetics in medicine : official journal of the American College of Medical Genetics. 23(10):1933-1943

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