CRISPR

CRISPR7-npas4l

ID
ZDB-CRISPR-230227-1
Name
CRISPR7-npas4l
Previous Names
None
Target
Sequence
5' - CCAGAGCCACTGCTGGACGAGGG - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "GGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
bns313Tg npas4l
Expression
Gene expression in Wild Types + CRISPR7-npas4l
No data available
Phenotype
Phenotype resulting from CRISPR7-npas4l
No data available
Phenotype of all Fish created by or utilizing CRISPR7-npas4l
Phenotype Fish Conditions Figures
embryonic blood vessel endothelial progenitor cell endothelial cell migration decreased process quality, abnormal lmo2bns499/bns499; npas4lbns313Tg; c264Tg; y1Tg standard conditions Fig. 6 with image from Mattonet et al., 2022
pronephros pax2a expression increased amount, abnormal lmo2bns499/bns499; npas4lbns313Tg; c264Tg; y1Tg standard conditions Fig. 6 with image from Mattonet et al., 2022
skeletal muscle pax2a expression increased amount, abnormal npas4lbns313Tg; etsrpbns426/bns426; c264Tg; y1Tg standard conditions Fig. 6 with image from Mattonet et al., 2022
embryonic blood vessel endothelial progenitor cell endothelial cell fate specification decreased process quality, abnormal npas4lbns313Tg; etsrpbns426/bns426; c264Tg; y1Tg standard conditions Fig. 6 with image from Mattonet et al., 2022
pronephros pax2a expression decreased amount, abnormal npas4lbns313Tg; etsrpbns426/bns426; c264Tg; y1Tg standard conditions Fig. 6 with image from Mattonet et al., 2022
embryonic blood vessel endothelial progenitor cell endothelial cell migration decreased process quality, abnormal tal1bns498/bns498; npas4lbns313Tg; c264Tg; y1Tg standard conditions Fig. 6 with image from Mattonet et al., 2022
pronephros pax2a expression increased amount, abnormal tal1bns498/bns498; npas4lbns313Tg; c264Tg; y1Tg standard conditions Fig. 6 with image from Mattonet et al., 2022
Citations
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.