CRISPR

CRISPR2-rab11fip5a

ID

ZDB-CRISPR-230215-2

Name

CRISPR2-rab11fip5a

Previous Names

None

Target

rab11fip5a (1)

Sequence

5' - AAACTCGCGCGCAACCCTCGGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The first three "A"s do not match the sequence in Ensembl.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
co35	rab11fip5a
co38	rab11fip5a

Expression

Gene expression in Wild Types + CRISPR2-rab11fip5a

No data available

Phenotype

Phenotype resulting from CRISPR2-rab11fip5a

No data available

Phenotype of all Fish created by or utilizing CRISPR2-rab11fip5a

Phenotype	Fish	Conditions	Figures
intestine accumulation intestine intracellular vesicle, abnormal	rab11fip5a^co38/co38	standard conditions	Fig. 3. from Jewett et al., 2020
intestinal epithelial cell subapical part of cell accumulation intestinal epithelial cell intracellular vesicle, abnormal	rab11fip5a^co38/co38	standard conditions	Fig. 3. from Jewett et al., 2020
mid intestine microvillus decreased length, abnormal	rab11fip5a^co38/co38	standard conditions	Fig. 3. from Jewett et al., 2020
intestinal epithelial cell keratin filament mislocalised, abnormal	rab11fip5a^co38/co38	standard conditions	Fig. 3. from Jewett et al., 2020
terminal web assembly process quality, abnormal	rab11fip5a^co38/co38	standard conditions	Fig. 3. from Jewett et al., 2020
intestine terminal web structure, abnormal	rab11fip5a^co38/co38	standard conditions	Fig. 3. from Jewett et al., 2020
whole organism dead, abnormal	rab11fip5a^co35/co35; rab11fip5b^co40/co40	standard conditions	text only from Jewett et al., 2020
mid intestine microvillus morphology, exacerbated	rab11fip5a^co35/co35; rab11fip5b^co40/co40	standard conditions	Fig. 4. from Jewett et al., 2020
enterocyte inclusion body increased amount, abnormal	rab11fip5a^co35/co35; rab11fip5b^co40/co40	standard conditions	Fig. 4. from Jewett et al., 2020
mid intestine microvillus decreased amount, abnormal	rab11fip5a^co35/co35; rab11fip5b^co40/co40	standard conditions	Fig. 4. from Jewett et al., 2020
intestine terminal web structure, abnormal	rab11fip5a^co35/co35; rab11fip5b^co40/co40	standard conditions	Fig. 4. from Jewett et al., 2020
kidney lumen increased amount, abnormal	rab11fip5a^co35/co35; rab11fip5b^co40/co40	standard conditions	Fig. 4. from Jewett et al., 2020
intestinal epithelial cell subapical part of cell accumulation intestinal epithelial cell intracellular vesicle, abnormal	rab11fip5a^co35/co35; rab11fip5b^co40/co40	standard conditions	Fig. 4. from Jewett et al., 2020
mid intestine microvillus decreased length, abnormal	rab11fip5a^co35/co35; rab11fip5b^co40/co40	standard conditions	Fig. 4. from Jewett et al., 2020
intestinal epithelial cell keratin filament mislocalised, abnormal	rab11fip5a^co35/co35; rab11fip5b^co40/co40	standard conditions	Fig. 4. from Jewett et al., 2020
intestinal epithelial cell accumulation intestine intracellular vesicle, abnormal	rab11fip5a^co35/co35; rab11fip5b^co40/co40	standard conditions	text only from Jewett et al., 2020
terminal web assembly process quality, abnormal	rab11fip5a^co35/co35; rab11fip5b^co40/co40	standard conditions	Fig. 4. from Jewett et al., 2020
microvillus organization disrupted, abnormal	rab11fip5a^co35/co35; rab11fip5b^co40/co40	standard conditions	Fig. 4. from Jewett et al., 2020

Citations