CRISPR

CRISPR3-api5

ID

ZDB-CRISPR-230117-11

Name

CRISPR3-api5

Previous Names

Target

Sequence

5' - CGCACAGCTCGATCTGTGTG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

No data available

Expression

Gene expression in Wild Types + CRISPR3-api5

No data available

Phenotype

Phenotype resulting from CRISPR3-api5

Phenotype	Fish	Figures
heart malformed, abnormal	WIK + CRISPR3-api5	FIGURE 3 from Winter et al., 2022
pericardium edematous, abnormal	WIK + CRISPR3-api5	FIGURE 3 from Winter et al., 2022
whole organism malformed, abnormal	WIK + CRISPR3-api5	FIGURE 3 from Winter et al., 2022

Phenotype of all Fish created by or utilizing CRISPR3-api5

Phenotype	Fish	Conditions	Figures
cardiac ventricle increased volume, abnormal	WIK + CRISPR1-api5 + CRISPR2-api5 + CRISPR3-api5	standard conditions	FIGURE 4 from Winter et al., 2022
pericardium edematous, abnormal	WIK + CRISPR1-api5 + CRISPR2-api5 + CRISPR3-api5	standard conditions	FIGURE 4 from Winter et al., 2022
pericardium edematous, abnormal	WIK + CRISPR3-api5	standard conditions	FIGURE 3 from Winter et al., 2022
heart malformed, abnormal	WIK + CRISPR3-api5	standard conditions	FIGURE 3 from Winter et al., 2022
whole organism malformed, abnormal	WIK + CRISPR3-api5	standard conditions	FIGURE 3 from Winter et al., 2022

Citations