CRISPR

CRISPR4-bmp6

ID

ZDB-CRISPR-220926-4

Name

CRISPR4-bmp6

Previous Names

None

Target

bmp6 (1)

Sequence

5' - TTTGGCAGGTAGCAGTTCTGTAC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
hj2	bmp6

Expression

Gene expression in Wild Types + CRISPR4-bmp6

No data available

Phenotype

Phenotype resulting from CRISPR4-bmp6

No data available

Phenotype of all Fish created by or utilizing CRISPR4-bmp6

Phenotype	Fish	Conditions	Figures
whole organism sp7 expression decreased amount, abnormal	bmp6^hj2/hj2	standard conditions	Fig. 9 from Xu et al., 2022
canonical NF-kappaB signal transduction increased process quality, abnormal	bmp6^hj2/hj2	standard conditions	Fig. 5 from Xu et al., 2022
regulation of tumor necrosis factor production increased process quality, abnormal	bmp6^hj2/hj2	standard conditions	Fig. 5, Fig. 7 from Xu et al., 2022
whole organism sik1 expression increased amount, abnormal	bmp6^hj2/hj2	standard conditions	Fig. 7, Fig. 8 from Xu et al., 2022
whole organism runx2a expression decreased amount, abnormal	bmp6^hj2/hj2	standard conditions	Fig. 9 from Xu et al., 2022
intermuscular bone absent, abnormal	bmp6^hj2/hj2	standard conditions	Fig. 2 from Xu et al., 2022
regulation of osteoblast differentiation decreased process quality, abnormal	bmp6^hj2/hj2	standard conditions	Fig. 9 from Xu et al., 2022
regulation of osteoclast differentiation decreased process quality, abnormal	bmp6^hj2/hj2	standard conditions	Fig. 5 from Xu et al., 2022
whole organism dlx5a expression decreased amount, abnormal	bmp6^hj2/hj2	standard conditions	Fig. 9 from Xu et al., 2022
regulation of bone development increased process quality, abnormal	bmp6^hj2/hj2	standard conditions	Fig. 7 from Xu et al., 2022
BMP signaling pathway decreased process quality, abnormal	bmp6^hj2/hj2	standard conditions	Fig. 5 from Xu et al., 2022
regulation of bone development decreased process quality, abnormal	bmp6^hj2/hj2	standard conditions	Fig. 5 from Xu et al., 2022
whole organism alpl expression decreased amount, abnormal	bmp6^hj2/hj2	standard conditions	Fig. 9 from Xu et al., 2022
myoseptum osteoblast GFP expression decreased amount, abnormal	bmp6^hj2/hj2; cy25Tg/cy25Tg	standard conditions	Fig. 6 from Xu et al., 2022
myoseptum osteoclast GFP expression increased amount, abnormal	bmp6^hj2/hj2; cy25Tg/cy25Tg	standard conditions	Fig. 6 from Xu et al., 2022
myoseptum osteoblast acp5b expression decreased amount, abnormal	bmp6^hj2/hj2; cy25Tg/cy25Tg	standard conditions	Fig. 6 from Xu et al., 2022
myoseptum osteoclast acp5b expression increased amount, abnormal	bmp6^hj2/hj2; cy25Tg/cy25Tg	standard conditions	Fig. 6 from Xu et al., 2022

Citations