CRISPR

CRISPR1-ly96

ID

ZDB-CRISPR-220328-5

Name

CRISPR1-ly96

Previous Names

None

Target

ly96 (1)

Sequence

5' - GGGTATCAGATATGGCGCTT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
b1364	ly96
b1365	ly96
b1366	ly96

Expression

Gene expression in Wild Types + CRISPR1-ly96

No data available

Phenotype

Phenotype resulting from CRISPR1-ly96

No data available

Phenotype of all Fish created by or utilizing CRISPR1-ly96

Phenotype	Fish	Conditions	Figures
whole organism increased life span, abnormal	ly96^b1364/b1364 (AB)	chemical treatment by injection: lipopolysaccharide	Fig. 6 from Loes et al., 2021
whole organism dead, abnormal	ly96^b1364/b1364 (AB)	chemical treatment by injection: lipopolysaccharide	Fig. 6 from Loes et al., 2021
whole organism response to lipopolysaccharide decreased process quality, abnormal	ly96^b1364/b1364 (AB)	chemical treatment by injection: lipopolysaccharide	Fig. 5 from Loes et al., 2021
whole organism regulation of cytokine production involved in immune response decreased process quality, abnormal	ly96^b1364/b1364 (AB)	chemical treatment by injection: lipopolysaccharide	Fig. 5 from Loes et al., 2021
whole organism il1b expression decreased amount, abnormal	ly96^b1365/b1365 (AB)	control	Fig. 5 from Loes et al., 2021
whole organism il1b expression increased amount, abnormal	ly96^b1365/b1365 (AB)	chemical treatment by injection: lipopolysaccharide	Fig. 5 from Loes et al., 2021
whole organism dead, abnormal	ly96^b1365/b1365 (AB)	chemical treatment by injection: lipopolysaccharide	Fig. 6 from Loes et al., 2021
whole organism mmp9 expression decreased amount, abnormal	ly96^b1365/b1365 (AB)	control	Fig. 5 from Loes et al., 2021
whole organism mmp9 expression increased amount, abnormal	ly96^b1365/b1365 (AB)	chemical treatment by injection: lipopolysaccharide	Fig. 5 from Loes et al., 2021
whole organism il1b expression increased amount, abnormal	ly96^b1366/b1366 (AB)	chemical treatment by injection: lipopolysaccharide	Fig. 5 from Loes et al., 2021
whole organism decreased life span, abnormal	ly96^b1366/b1366 (AB)	chemical treatment by injection: lipopolysaccharide	Fig. 6 from Loes et al., 2021
whole organism dead, abnormal	ly96^b1366/b1366 (AB)	chemical treatment by injection: lipopolysaccharide	Fig. 6 from Loes et al., 2021
whole organism mmp9 expression increased amount, abnormal	ly96^b1366/b1366 (AB)	chemical treatment by injection: lipopolysaccharide	Fig. 5 from Loes et al., 2021

Citations