CRISPR

CRISPR2-bach1a

ID

ZDB-CRISPR-220106-2

Name

CRISPR2-bach1a

Previous Names

None

Target

Sequence

5' - CTGCGCAGCGCTGTCGCAAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
stl663	bach1a

Expression

Gene expression in Wild Types + CRISPR2-bach1a

No data available

Phenotype

Phenotype resulting from CRISPR2-bach1a

No data available

Phenotype of all Fish created by or utilizing CRISPR2-bach1a

Phenotype	Fish	Conditions	Figures
swimming behavior decreased process quality, abnormal	TU + CRISPR1-bach1a + CRISPR1-bach1b + CRISPR2-bach1a + CRISPR2-bach1b	transection: spinal cord, water flow	Fig. 4 from Klatt Shaw et al., 2021
regenerating tissue glial cell proliferation decreased process quality, abnormal	TU + CRISPR1-bach1a + CRISPR1-bach1b + CRISPR2-bach1a + CRISPR2-bach1b	transection: spinal cord	Fig. 4 from Klatt Shaw et al., 2021
spinal cord regeneration decreased process quality, abnormal	TU + CRISPR1-bach1a + CRISPR1-bach1b + CRISPR2-bach1a + CRISPR2-bach1b	transection: spinal cord	Fig. 4 from Klatt Shaw et al., 2021
regenerating tissue glial cell decreased amount, abnormal	TU + CRISPR1-bach1a + CRISPR1-bach1b + CRISPR2-bach1a + CRISPR2-bach1b	transection: spinal cord	Fig. 4 from Klatt Shaw et al., 2021
regenerating tissue glial cell gfap expression decreased amount, abnormal	TU + CRISPR1-bach1a + CRISPR1-bach1b + CRISPR2-bach1a + CRISPR2-bach1b	transection: spinal cord	Fig. 4 from Klatt Shaw et al., 2021

Citations