CRISPR

CRISPR1-lss

ID

ZDB-CRISPR-210319-7

Name

CRISPR1-lss

Previous Names

None

Target

lss (1)

Sequence

5' - GGACAGACCGCAGAGCATGC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
nu60	lss

Expression

Gene expression in Wild Types + CRISPR1-lss

No data available

Phenotype

Phenotype resulting from CRISPR1-lss

No data available

Phenotype of all Fish created by or utilizing CRISPR1-lss

Phenotype	Fish	Conditions	Figures
whole organism decreased life span, abnormal	lss^nu60/nu60	standard conditions	Fig. 3. from Anderson et al., 2020
whole organism dead, abnormal	lss^nu60/nu60	standard conditions	Fig. 3. from Anderson et al., 2020
whole organism cholesterol decreased amount, abnormal	lss^nu60/nu60	standard conditions	text only from Anderson et al., 2020
whole organism decreased life span, abnormal	lss^nu60/+; msmo1^nu81/nu81	standard conditions	Fig. 3. from Anderson et al., 2020
whole organism dead, abnormal	lss^nu60/+; msmo1^nu81/nu81	standard conditions	Fig. 3. from Anderson et al., 2020
whole organism embryo development delayed, abnormal	lss^nu60/nu60; msmo1^nu81/nu81	standard conditions	text only from Anderson et al., 2020
pterotic chondrocyte differentiation decreased process quality, abnormal	lss^nu60/nu60; nu101Tg	standard conditions	Fig. 5. from Anderson et al., 2020
endochondral bone ossification increased process quality, abnormal	lss^nu60/nu60; nu101Tg	standard conditions	Fig. 4. from Anderson et al., 2020
hypural condensed, abnormal	lss^nu60/nu60; nu101Tg	standard conditions	Fig. 4. from Anderson et al., 2020
ceratohyal bone condensed, abnormal	lss^nu60/nu60; nu101Tg	standard conditions	Fig. 4. from Anderson et al., 2020
pterotic hypertrophic chondrocyte msmo1 expression increased distribution, abnormal	lss^nu60/nu60; nu101Tg	standard conditions	Fig. 5. from Anderson et al., 2020
hyomandibula condensed, abnormal	lss^nu60/nu60; nu101Tg	standard conditions	Fig. 4. from Anderson et al., 2020
pterotic columnar chondrocyte msmo1 expression increased distribution, abnormal	lss^nu60/nu60; nu101Tg	standard conditions	Fig. 5. from Anderson et al., 2020
postcranial axial skeleton ossification increased process quality, abnormal	lss^nu60/nu60; nu101Tg	standard conditions	Fig. 3. from Anderson et al., 2020
cranium ossification increased process quality, abnormal	lss^nu60/nu60; nu101Tg	standard conditions	Fig. 3. from Anderson et al., 2020
whole organism decreased length, abnormal	lss^nu60/nu60; nu101Tg	standard conditions	Fig. 3. from Anderson et al., 2020
pterotic chondrocyte col2a1a expression increased distribution, abnormal	lss^nu60/nu60; nu101Tg	standard conditions	Fig. 5. from Anderson et al., 2020
postcranial axial skeleton malformed, abnormal	lss^nu60/nu60; nu101Tg	standard conditions	Fig. 3. from Anderson et al., 2020
hypural deformed, abnormal	lss^nu60/nu60; nu101Tg	standard conditions	Fig. 4. from Anderson et al., 2020
hyomandibula deformed, abnormal	lss^nu60/nu60; nu101Tg	standard conditions	Fig. 4. from Anderson et al., 2020
cranium malformed, abnormal	lss^nu60/nu60; nu101Tg	standard conditions	Fig. 3. from Anderson et al., 2020
ceratohyal bone deformed, abnormal	lss^nu60/nu60; nu101Tg	standard conditions	Fig. 4. from Anderson et al., 2020
head decreased size, abnormal	lss^nu60/nu60; nu101Tg	standard conditions	Fig. 3. from Anderson et al., 2020
pterotic growth plate cartilage morphogenesis decreased process quality, abnormal	lss^nu60/nu60; nu101Tg	standard conditions	Fig. 5. from Anderson et al., 2020

Citations