CRISPR

CRISPR1-polr2d

ID

ZDB-CRISPR-210217-1

Name

CRISPR1-polr2d

Previous Names

None

Target

polr2d (1)

Sequence

5' - GCAGCAGAACGAGAGCGCGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
agu8	polr2d

Expression

Gene expression in Wild Types + CRISPR1-polr2d

No data available

Phenotype

Phenotype resulting from CRISPR1-polr2d

No data available

Phenotype of all Fish created by or utilizing CRISPR1-polr2d

Phenotype	Fish	Conditions	Figures
whole organism dnd1 expression increased amount, abnormal	polr2d^agu8/agu8 (AB)	standard conditions	Fig. 4 from Maeta et al., 2020
eye decreased size, abnormal	polr2d^agu8/agu8 (AB)	standard conditions	Fig. 1 from Maeta et al., 2020
pericardium edematous, abnormal	polr2d^agu8/agu8 (AB)	standard conditions	Fig. 1 from Maeta et al., 2020
whole organism dead, abnormal	polr2d^agu8/agu8 (AB)	standard conditions	text only from Maeta et al., 2020
whole organism ddx4 expression increased amount, abnormal	polr2d^agu8/agu8 (AB)	standard conditions	Fig. 4 from Maeta et al., 2020
whole organism glra4a expression decreased amount, abnormal	polr2d^agu8/agu8 (AB)	standard conditions	Fig. 4 from Maeta et al., 2020
whole organism hypoplastic, abnormal	polr2d^agu8/agu8 (AB)	standard conditions	Fig. 1 from Maeta et al., 2020
head cell death increased occurrence, abnormal	polr2d^agu8/agu8 (AB)	control	Fig. 3 from Maeta et al., 2020
whole organism krt4 expression decreased amount, abnormal	polr2d^agu8/agu8 (AB)	standard conditions	Fig. 4 from Maeta et al., 2020
trunk cell death increased occurrence, abnormal	polr2d^agu8/agu8 (AB)	control	Fig. 3 from Maeta et al., 2020
whole organism polr2d expression decreased amount, abnormal	polr2d^agu8/agu8 (AB)	standard conditions	Fig. 4 from Maeta et al., 2020
whole organism rpl13a expression decreased amount, abnormal	polr2d^agu8/agu8 (AB)	standard conditions	Fig. 4 from Maeta et al., 2020
somite decreased amount, abnormal	polr2d^agu8/agu8 (AB)	standard conditions	Fig. 2 from Maeta et al., 2020

Citations