CRISPR

CRISPR3-znf143a

ID
ZDB-CRISPR-200622-3
Name
CRISPR3-znf143a
Previous Names
None
Target
Sequence
5' - GAAACTAACGTTACACGCCT - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
This CRISPR was designed to target the first intronic region of znf143a. The PAM site was "GGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
No data available
Expression
Gene expression in Wild Types + CRISPR3-znf143a
No data available
Phenotype
Phenotype resulting from CRISPR3-znf143a
No data available
Phenotype of all Fish created by or utilizing CRISPR3-znf143a
Phenotype Fish Conditions Figures
brain development disrupted, abnormal AB/TL + CRISPR1-znf143a + CRISPR2-znf143a + CRISPR3-znf143a standard conditions Fig. 5 with image from Huning et al., 2020
whole organism znf143a expression decreased amount, abnormal AB/TL + CRISPR1-znf143a + CRISPR2-znf143a + CRISPR3-znf143a standard conditions Fig. 6 with image from Huning et al., 2020
hindbrain malformed, abnormal AB/TL + CRISPR1-znf143a + CRISPR2-znf143a + CRISPR3-znf143a standard conditions Fig. 5 with image from Huning et al., 2020
brain morphology, abnormal AB/TL + CRISPR1-znf143a + CRISPR2-znf143a + CRISPR3-znf143a standard conditions Fig. 5 with image from Huning et al., 2020
hindbrain increased size, abnormal AB/TL + CRISPR1-znf143a + CRISPR2-znf143a + CRISPR3-znf143a standard conditions Fig. 5 with image from Huning et al., 2020
whole organism znf143b expression increased amount, abnormal AB/TL + CRISPR1-znf143a + CRISPR2-znf143a + CRISPR3-znf143a standard conditions Fig. 6 with image from Huning et al., 2020
brain disorganized, abnormal AB/TL + CRISPR1-znf143a + CRISPR2-znf143a + CRISPR3-znf143a standard conditions Fig. 5 with image from Huning et al., 2020
midbrain malformed, abnormal AB/TL + CRISPR1-znf143a + CRISPR2-znf143a + CRISPR3-znf143a standard conditions Fig. 5 with image from Huning et al., 2020
Citations
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