CRISPR

CRISPR1-ciao3

ID
ZDB-CRISPR-200103-1
Name
CRISPR1-ciao3
Previous Names
  • CRISPR1-narfl
  • CRISPR3-ciao3
  • CRISPR3-narfl
Target
Sequence
5' - ATGTTGGCGTCTGCATGTCC - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "AGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
ihb379 ciao3
Expression
Gene expression in Wild Types + CRISPR1-ciao3
No data available
Phenotype
Phenotype resulting from CRISPR1-ciao3
No data available
Phenotype of all Fish created by or utilizing CRISPR1-ciao3
Phenotype Fish Conditions Figures
brain endothelial blood brain barrier broken, abnormal ciao3ihb379/ihb379 standard conditions Figure 1 with image from Hu et al., 2025
whole organism kdr expression increased amount, abnormal ciao3ihb379/ihb379 standard conditions Fig. 5 from Luo et al., 2019
intestine fabp1a expression spatial pattern, abnormal ciao3ihb379/ihb379 standard conditions Fig. 3 from Luo et al., 2019
whole organism dead, abnormal ciao3ihb379/ihb379 control Fig. S2,  Figure 3 with image from Hu et al., 2025
intestine fabp1a expression decreased amount, abnormal ciao3ihb379/ihb379 standard conditions Fig. 3 from Luo et al., 2019
whole organism bmp7b expression increased amount, abnormal ciao3ihb379/ihb379 standard conditions Fig. 5 from Luo et al., 2019
whole organism cellular respiration decreased process quality, abnormal ciao3ihb379/ihb379 standard conditions Figure 2 with image from Hu et al., 2025
whole organism Endothelin-1 increased amount, abnormal ciao3ihb379/ihb379 standard conditions Figure 2 with image from Hu et al., 2025
whole organism aconitate hydratase activity decreased process quality, abnormal ciao3ihb379/ihb379 standard conditions Figure 2 with image from Hu et al., 2025
whole organism lipid oxidation increased process quality, abnormal ciao3ihb379/ihb379 standard conditions Figure 2 with image,  Figure 3 with image from Hu et al., 2025
whole organism cyp2n13 expression decreased amount, abnormal ciao3ihb379/ihb379 standard conditions Figure 2 with image from Hu et al., 2025
whole organism lipid increased amount, abnormal ciao3ihb379/ihb379 standard conditions Figure 2 with image from Hu et al., 2025
whole organism gck expression increased amount, abnormal ciao3ihb379/ihb379 standard conditions Fig. 5 from Luo et al., 2019
whole organism reactive oxygen species increased amount, abnormal ciao3ihb379/ihb379 standard conditions Figure 2 with image from Hu et al., 2025
whole organism deformed, abnormal ciao3ihb379/ihb379 standard conditions Fig. S2 from Hu et al., 2025
whole organism cyp8b1.2 expression decreased amount, abnormal ciao3ihb379/ihb379 standard conditions Figure 2 with image from Hu et al., 2025
blood vessel development hypertrophic growth, abnormal ciao3ihb379/ihb379 standard conditions Fig. 5 from Luo et al., 2019
whole organism tek expression increased amount, abnormal ciao3ihb379/ihb379 standard conditions Fig. 5 from Luo et al., 2019
whole organism bmp16 expression increased amount, abnormal ciao3ihb379/ihb379 standard conditions Fig. 5 from Luo et al., 2019
whole organism lipid oxidation process characteristic, ameliorated ciao3ihb379/ihb379 chemical treatment by environment: ferrostatin-1 Figure 3 with image from Hu et al., 2025
whole organism cyp2x7 expression increased amount, abnormal ciao3ihb379/ihb379 standard conditions Figure 2 with image from Hu et al., 2025
glutathione synthase activity decreased efficacy, abnormal ciao3ihb379/ihb379 standard conditions Fig. 4 from Luo et al., 2019
exocrine pancreas fabp2 expression spatial pattern, abnormal ciao3ihb379/ihb379 standard conditions Fig. 3 from Luo et al., 2019
blood vessel cyp2p8 expression decreased distribution, abnormal ciao3ihb379/ihb379 standard conditions Figure 2 with image from Hu et al., 2025
whole organism bmp2b expression increased amount, abnormal ciao3ihb379/ihb379 standard conditions Fig. 5 from Luo et al., 2019
whole organism cyp3c3 expression decreased amount, abnormal ciao3ihb379/ihb379 standard conditions Figure 2 with image from Hu et al., 2025
whole organism nitric oxide decreased amount, abnormal ciao3ihb379/ihb379 standard conditions Figure 2 with image from Hu et al., 2025
whole organism cyp2p8 expression decreased amount, abnormal ciao3ihb379/ihb379 standard conditions Figure 2 with image from Hu et al., 2025
aconitate hydratase activity decreased efficacy, abnormal ciao3ihb379/ihb379 standard conditions Fig. 1 from Luo et al., 2019
adenylyl-sulfate reductase (glutathione) activity decreased efficacy, abnormal ciao3ihb379/ihb379 standard conditions Fig. 4 from Luo et al., 2019
whole organism iron cation increased amount, abnormal ciao3ihb379/ihb379 standard conditions Figure 2 with image from Hu et al., 2025
whole organism viability, ameliorated ciao3ihb379/ihb379 chemical treatment by environment: ferrostatin-1 Figure 3 with image from Hu et al., 2025
whole organism glutathione decreased amount, abnormal ciao3ihb379/ihb379 standard conditions Figure 2 with image from Hu et al., 2025
blood vessel cyp2p8 expression spatial pattern, ameliorated ciao3ihb379/ihb379 chemical treatment by environment: Ophiopogonin D Figure 2 with image from Hu et al., 2025
superoxide dismutase activity decreased efficacy, abnormal ciao3ihb379/ihb379 standard conditions Fig. 4 from Luo et al., 2019
whole organism hkdc1 expression increased amount, abnormal ciao3ihb379/ihb379 standard conditions Fig. 5 from Luo et al., 2019
liver ela2l expression spatial pattern, abnormal ciao3ihb379/ihb379 standard conditions Fig. 3 from Luo et al., 2019
whole organism hif1ab expression increased amount, abnormal ciao3ihb379/ihb379 standard conditions Fig. 4 from Luo et al., 2019
brain rough endoplasmic reticulum morphology, abnormal ciao3ihb379/ihb379 standard conditions Figure 1 with image from Hu et al., 2025
blood vessel development hypertrophic growth, ameliorated ciao3ihb379/ihb379 chemical treatment: 2-methoxy-17beta-estradiol Fig. 5 from Luo et al., 2019
intestine hif1ab expression increased distribution, abnormal ciao3ihb379/ihb379 standard conditions Fig. 4 from Luo et al., 2019
whole organism cyp2k8 expression increased amount, abnormal ciao3ihb379/ihb379 standard conditions Figure 2 with image from Hu et al., 2025
digestive system compound organ morphology, abnormal ciao3ihb379/ihb379 standard conditions Fig. 3 from Luo et al., 2019
liver ela2l expression decreased amount, abnormal ciao3ihb379/ihb379 standard conditions Fig. 3 from Luo et al., 2019
swimming behavior increased process quality, abnormal ciao3ihb379/ihb379 control Figure 1 with image from Hu et al., 2025
exocrine pancreas fabp2 expression decreased amount, abnormal ciao3ihb379/ihb379 standard conditions Fig. 3 from Luo et al., 2019
whole organism pdgfrb expression increased amount, abnormal ciao3ihb379/ihb379 standard conditions Fig. 5 from Luo et al., 2019
whole organism angpt1 expression increased amount, abnormal ciao3ihb379/ihb379 standard conditions Fig. 5 from Luo et al., 2019
whole organism vegfab expression increased amount, abnormal ciao3ihb379/ihb379 standard conditions Fig. 5 from Luo et al., 2019
whole organism glutamine decreased amount, abnormal ciao3ihb379/ihb379 standard conditions Figure 2 with image from Hu et al., 2025
whole organism aldoca expression increased amount, abnormal ciao3ihb379/ihb379 standard conditions Fig. 5 from Luo et al., 2019
swimming behavior increased process quality, abnormal ciao3ihb379/+ control Figure 1 with image from Hu et al., 2025
whole organism nitric oxide decreased amount, abnormal ciao3ihb379/ihb379; s843Tg standard conditions Figure 2 with image from Hu et al., 2025
dorsal longitudinal anastomotic vessel disorganized, abnormal ciao3ihb379/ihb379; s843Tg standard conditions Figure 1 with image from Hu et al., 2025
dorsal aorta deformed, abnormal ciao3ihb379/ihb379; s843Tg standard conditions Figure 1 with image from Hu et al., 2025
lateral dorsal aorta blood circulation increased rate of continuous process, abnormal ciao3ihb379/ihb379; s843Tg standard conditions Figure 2 with image from Hu et al., 2025
posterior cardinal vein deformed, abnormal ciao3ihb379/ihb379; s843Tg standard conditions Figure 1 with image from Hu et al., 2025
brain endothelial blood brain barrier broken, abnormal ciao3ihb379/ihb379; s843Tg standard conditions Figure 1 with image from Hu et al., 2025
dorsal longitudinal anastomotic vessel decreased amount, abnormal ciao3ihb379/ihb379; s843Tg standard conditions Figure 1 with image from Hu et al., 2025
whole organism Endothelin-1 increased amount, abnormal ciao3ihb379/ihb379; s843Tg standard conditions Figure 2 with image from Hu et al., 2025
blood vessel development hypertrophic growth, abnormal ciao3ihb379/ihb379; y1Tg standard conditions Fig. 2 from Luo et al., 2019
Citations
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