CRISPR

CRISPR3-znfl1

ID

ZDB-CRISPR-190711-6

Name

CRISPR3-znfl1

Previous Names

Targets

Sequence

5' - GCACTGTCCTGTACTTCCCA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

No data available

Expression

Gene expression in Wild Types + CRISPR3-znfl1

No data available

Phenotype

Phenotype resulting from CRISPR3-znfl1

No data available

Phenotype of all Fish created by or utilizing CRISPR3-znfl1

Phenotype	Fish	Conditions	Figures
determination of left/right symmetry disrupted, abnormal	WT + CRISPR1-znfl1 + CRISPR2-znfl1 + CRISPR3-znfl1	standard conditions	Fig. 2 from Li et al., 2018
rhombomere 7 decreased distance somite 1, abnormal	WT + CRISPR1-znfl1 + CRISPR2-znfl1 + CRISPR3-znfl1	standard conditions	Fig. 1 from Li et al., 2018
lateral plate mesoderm left side spaw expression absent, abnormal	WT + CRISPR1-znfl1 + CRISPR2-znfl1 + CRISPR3-znfl1	standard conditions	Fig. 2 from Li et al., 2018
rhombomere 6 decreased distance somite 1, abnormal	WT + CRISPR1-znfl1 + CRISPR2-znfl1 + CRISPR3-znfl1	standard conditions	Fig. 1 from Li et al., 2018
lateral plate mesoderm left side pitx2 expression absent, abnormal	WT + CRISPR1-znfl1 + CRISPR2-znfl1 + CRISPR3-znfl1	standard conditions	Fig. 2 from Li et al., 2018
heart primordium lft2 expression absent, abnormal	WT + CRISPR1-znfl1 + CRISPR2-znfl1 + CRISPR3-znfl1	standard conditions	Fig. 2 from Li et al., 2018
diencephalon left side lft1 expression absent, abnormal	WT + CRISPR1-znfl1 + CRISPR2-znfl1 + CRISPR3-znfl1	standard conditions	Fig. 2 from Li et al., 2018

Citations