CRISPR

CRISPR2-ush2a

ID

ZDB-CRISPR-181023-1

Name

CRISPR2-ush2a

Previous Names

None

Target

ush2a (1)

Sequence

5' - ACAGTGTGAGTGCAAAGC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
rmc1	ush2a

Expression

Gene expression in Wild Types + CRISPR2-ush2a

No data available

Phenotype

Phenotype resulting from CRISPR2-ush2a

No data available

Phenotype of all Fish created by or utilizing CRISPR2-ush2a

Phenotype	Fish	Conditions	Figures
photoreceptor cell periciliary membrane compartment Ab1-whrna labeling decreased amount, abnormal	ush2a^rmc1/rmc1	standard conditions	Fig. 5 from Dona et al., 2018
photoreceptor cell periciliary membrane compartment ush2a expression absent, abnormal	ush2a^rmc1/rmc1	standard conditions	Fig. 4 from Dona et al., 2018
retinal outer nuclear layer apoptotic process increased occurrence, abnormal	ush2a^rmc1/rmc1	constant light, high light intensity	Fig. 6 from Dona et al., 2018
retina transmission of nerve impulse process quality, abnormal	ush2a^rmc1/rmc1	control	Fig. 7 from Dona et al., 2018
photoreceptor cell periciliary membrane compartment ab1-adgrv1 labeling decreased amount, abnormal	ush2a^rmc1/rmc1	standard conditions	Fig. S5 from Dona et al., 2018
retinal outer nuclear layer apoptotic process increased occurrence, abnormal	ush2a^rmc1/rmc1	control	Fig. 6 from Dona et al., 2018
photoreceptor cell periciliary membrane compartment Ab1-whrnb labeling decreased amount, abnormal	ush2a^rmc1/rmc1	standard conditions	Fig. 5 from Dona et al., 2018
photoreceptor cell ab1-ush2a labeling absent, abnormal	ush2a^rmc1/rmc1	control	Fig. 6 from Slijkerman et al., 2018
whole organism Ab9-map1lc3b labeling increased amount, abnormal	ush2a^rmc1/rmc1 (TL)	light duration	Figure 10 from Toms et al., 2020
eye photoreceptor cell periciliary membrane compartment ab1-ush2a labeling absent, abnormal	ush2a^rmc1/rmc1 (TL)	control	Fig. 2 from Dulla et al., 2021
visual perception decreased process quality, abnormal	ush2a^rmc1/rmc1 (TL)	standard conditions	Fig. 5 from Reurink et al., 2022
eye photoreceptor cell periciliary membrane compartment ush2a expression absent, abnormal	ush2a^rmc1/rmc1 (TL)	standard conditions	Fig. 3 from Reurink et al., 2022
whole organism Ab6-sqstm1 labeling increased amount, abnormal	ush2a^rmc1/rmc1 (TL)	light duration	Figure 10 from Toms et al., 2020
whole organism Ab9-map1lc3b labeling increased amount, abnormal	ush2a^rmc1/rmc1 (TL)	standard conditions	Figure 10 from Toms et al., 2020
whole organism atg12 expression increased amount, abnormal	ush2a^rmc1/rmc1 (TL)	standard conditions	Figure 10 from Toms et al., 2020
whole organism atg5 expression increased amount, abnormal	ush2a^rmc1/rmc1 (TL)	standard conditions	Figure 10 from Toms et al., 2020
retinal rod cell cell body ab5-rho labeling mislocalised, abnormal	ush2a^rmc1/rmc1 (TL)	standard conditions	Fig. S7, Figure 10 from Toms et al., 2020
photoreceptor cell Ab9-map1lc3b labeling increased distribution, abnormal	ush2a^rmc1/rmc1 (TL)	standard conditions	Figure 10 from Toms et al., 2020
long single cone cell ab1-opn1sw2 labeling mislocalised, abnormal	ush2a^rmc1/rmc1 (TL)	standard conditions	Fig. S7 from Toms et al., 2020
whole organism Ab6-sqstm1 labeling increased amount, abnormal	ush2a^rmc1/rmc1 (TL)	standard conditions	Figure 10 from Toms et al., 2020
retinal rod cell cell body ab5-rho labeling mislocalised, abnormal	ush2a^rmc1/rmc1 (TL)	light duration	Figure 10 from Toms et al., 2020
whole organism ab1-becn1 labeling increased amount, abnormal	ush2a^rmc1/rmc1 (TL)	light duration	Figure 10 from Toms et al., 2020
whole organism ab1-becn1 labeling increased amount, abnormal	ush2a^rmc1/rmc1 (TL)	standard conditions	Figure 10 from Toms et al., 2020
retina phototransduction, visible light decreased process quality, abnormal	ush2a^rmc1/rmc1 (TL)	standard conditions	Fig. 2 from Dulla et al., 2021
eye photoreceptor cell periciliary membrane compartment ab1-ush2a labeling spatial pattern, ameliorated	ush2a^rmc1/rmc1 + MO2-ush2a + MO3-ush2a (TL)	control	Fig. 2 from Dulla et al., 2021
eye photoreceptor cell periciliary membrane compartment ab1-ush2a labeling position, ameliorated	ush2a^rmc1/rmc1 + MO2-ush2a + MO3-ush2a (TL)	control	Fig. 2 from Dulla et al., 2021

Citations