CRISPR

CRISPR3-alkal1

ID

ZDB-CRISPR-181015-5

Name

CRISPR3-alkal1

Previous Names

None

Target

alkal1 (1)

Sequence

5' - GGGTCCAGACGCGTCACCAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The first two "G"s were added to the 5' end to enhance binding.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ya341	alkal1

Expression

Gene expression in Wild Types + CRISPR3-alkal1

No data available

Phenotype

Phenotype resulting from CRISPR3-alkal1

No data available

Phenotype of all Fish created by or utilizing CRISPR3-alkal1

Phenotype	Fish	Conditions	Figures
whole organism alkal1 expression decreased amount, abnormal	alkal1^ya341/ya341 (AB)	standard conditions	Fig. 1 from Mo et al., 2017
eye has fewer parts of type iridophore, abnormal	alkal1^ya341/+; alkal2b^ya342/ya342 (AB)	standard conditions	Fig. 4 from Mo et al., 2017
iridophore differentiation decreased occurrence, abnormal	alkal1^ya341/+; alkal2b^ya342/ya342 (AB)	standard conditions	Fig. 4 from Mo et al., 2017
caudal fin has fewer parts of type iridophore, abnormal	alkal1^ya341/ya341; alkal2a^ya340/ya340 (AB)	standard conditions	Fig. 2 from Mo et al., 2017
iridophore differentiation decreased occurrence, abnormal	alkal1^ya341/ya341; alkal2b^ya342/+ (AB)	standard conditions	Fig. 4 from Mo et al., 2017
eye has fewer parts of type iridophore, abnormal	alkal1^ya341/ya341; alkal2b^ya342/+ (AB)	standard conditions	Fig. 4 from Mo et al., 2017
iridophore differentiation decreased occurrence, abnormal	alkal1^ya341/ya341; alkal2b^ya342/ya342 (AB)	standard conditions	Fig. 4 from Mo et al., 2017
eye lacks all parts of type iridophore, abnormal	alkal1^ya341/ya341; alkal2b^ya342/ya342 (AB)	standard conditions	Fig. 4 from Mo et al., 2017
whole organism alkal2b expression decreased amount, abnormal	alkal1^ya341/ya341; alkal2a^ya340/ya340; alkal2b^ya342/ya342 (AB)	standard conditions	Fig. 1 from Mo et al., 2017
caudal fin lacks all parts of type iridophore, abnormal	alkal1^ya341/ya341; alkal2a^ya340/ya340; alkal2b^ya342/ya342 (AB)	standard conditions	Fig. 2 from Mo et al., 2017
eye lacks all parts of type iridophore, abnormal	alkal1^ya341/ya341; alkal2a^ya340/ya340; alkal2b^ya342/ya342 (AB)	standard conditions	Fig. 2 from Mo et al., 2017
whole organism alkal2a expression decreased amount, abnormal	alkal1^ya341/ya341; alkal2a^ya340/ya340; alkal2b^ya342/ya342 (AB)	standard conditions	Fig. 1 from Mo et al., 2017
whole organism alkal1 expression decreased amount, abnormal	alkal1^ya341/ya341; alkal2a^ya340/ya340; alkal2b^ya342/ya342 (AB)	standard conditions	Fig. 1 from Mo et al., 2017

Citations