CRISPR

CRISPR2-hif1al

ID

ZDB-CRISPR-180620-1

Name

CRISPR2-hif1al

Previous Names

CRISPR1-hif3a (1)

Target

hif1al (1)

Sequence

5' - GGACAAAGCTGCCATCATGAGGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ihb20180620	hif1al

Expression

Gene expression in Wild Types + CRISPR2-hif1al

No data available

Phenotype

Phenotype resulting from CRISPR2-hif1al

No data available

Phenotype of all Fish created by or utilizing CRISPR2-hif1al

Phenotype	Fish	Conditions	Figures
erythroblast decreased amount, abnormal	hif1al^{ihb20180620/ihb20180620}	standard conditions	Fig. 2 from Cai et al., 2020
whole organism viability, exacerbated	hif1al^{ihb20180620/ihb20180620}	hypoxia	Fig. 1 from Cai et al., 2020
nucleate erythrocyte alas2 expression decreased amount, abnormal	hif1al^{ihb20180620/ihb20180620}	standard conditions	Fig. 3 from Cai et al., 2020
primitive erythrocyte differentiation decreased occurrence, abnormal	hif1al^{ihb20180620/ihb20180620}	standard conditions	Fig. 2 from Cai et al., 2020
nucleate erythrocyte gata1a expression decreased amount, abnormal	hif1al^{ihb20180620/ihb20180620}	standard conditions	Fig. 3 from Cai et al., 2020
basophilic erythroblast increased amount, abnormal	hif1al^{ihb20180620/ihb20180620}	standard conditions	Fig. 2 from Cai et al., 2020
response to hypoxia decreased efficacy, abnormal	hif1al^{ihb20180620/ihb20180620}	hypoxia	Fig. 1 from Cai et al., 2020
nucleate erythrocyte decreased amount, abnormal	hif1al^{ihb20180620/ihb20180620}	standard conditions	Fig. 2 from Cai et al., 2020
basophilic erythroblast decreased amount, abnormal	hif1al^{ihb20180620/ihb20180620}	standard conditions	Fig. 2 from Cai et al., 2020
nucleate erythrocyte slc4a1a expression decreased amount, abnormal	hif1al^{ihb20180620/ihb20180620}	standard conditions	Fig. 3 from Cai et al., 2020
erythrocyte development disrupted, abnormal	hif1al^{ihb20180620/ihb20180620}	standard conditions	Fig. 2 from Cai et al., 2020
nucleate erythrocyte hbae3 expression decreased amount, abnormal	hif1al^{ihb20180620/ihb20180620}	standard conditions	Fig. 3 from Cai et al., 2020

Citations