CRISPR

CRISPR2-mir34a, si:ch211-28e16.5

ID
ZDB-CRISPR-180504-8
Name
CRISPR2-mir34a, si:ch211-28e16.5
Previous Names
None
Targets
Sequence
5' - GGGTTGACTGGATGGATGAA - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
This CRISPR was designed to excise mir34a when paired with CRISPR1-mir34a, si:ch211-28e16.5 (ZDB-CRISPR-180504-7). If used on its own this CRISPR would only target si:ch211-28e16.5.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
hza51 mir34a
Expression
Gene expression in Wild Types + CRISPR2-mir34a, si:ch211-28e16.5
No data available
Phenotype
Phenotype resulting from CRISPR2-mir34a, si:ch211-28e16.5
No data available
Phenotype of all Fish created by or utilizing CRISPR2-mir34a, si:ch211-28e16.5
Phenotype Fish Conditions Figures
testis gsk3aa expression increased amount, abnormal mir34ahza51/hza51 standard conditions Fig. 6 from Guo et al., 2017
fertilization increased occurrence, abnormal mir34ahza51/hza51 standard conditions Fig. 5 from Guo et al., 2017
sperm increased cellular motility, abnormal mir34ahza51/hza51 standard conditions Fig. 4 with image from Guo et al., 2017
whole organism increased fertility, abnormal mir34ahza51/hza51 standard conditions Fig. 5 from Guo et al., 2017
Citations
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