MGC:77897

ID

ZDB-CDNA-040425-934

Name

MGC:77897

Symbol

MGC:77897

Previous Names

• FR_5586 (1)

Type

cDNA

Location

Unmapped

Genome Resources

None

Species

Danio rerio

Library

NIH_ZGC_7

Digest

Insert Size

1406

Cloning Site

NotI-SmaI

Vector Type

Plasmid

Vector

pExpress-1

Polymerase

T7 RNA polymerase

PCR Amplification

Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TAATACGACTCACTATAGGG 3'
5' GGATCCATTAACCCTCACTAAAGGGAAGTTTCGCTCCGGAATTCCCGGG 3'

Suppliers

None

Quality

( Simple to use, intense expression pattern restricted to a few structures )

Protocol

Thisse in situ hybridization protocol

Note

None