cDNA
MGC:77583
- ID
- ZDB-CDNA-040425-695
- Name
- MGC:77583
- Symbol
- MGC:77583
- Previous Names
-
- FR_5720 (1)
- Type
- cDNA
- Location
- Unmapped
- Genome Resources
- None
- Species
- Danio rerio
- Library
- NIH_ZGC_10
- Digest
- Insert Size
- 1743
- Cloning Site
- NotI-SmaI
- Vector Type
- Plasmid
- Vector
- pExpress-1
- Polymerase
- T7 RNA polymerase
- PCR Amplification
-
Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TAATACGACTCACTATAGGG 3'
5' GGATCCATTAACCCTCACTAAAGGGAAGTTTCGCTCCGGAATTCCCGGG 3' - Suppliers
- None
- Quality
- ( Probe is difficult to use. Generally basal level of expression with more intense labeling in particular structure. )
- Protocol
- Thisse in situ hybridization protocol
- Note
- None
Gene Expression
- Directly Submitted Expression Data
- 1 figure (1 image) from Thisse et al., 2004 [MGC:77583]
Marker Relationships
Sequences
Citations