MGC:73031

ID

ZDB-CDNA-040425-426

Name

MGC:73031

Symbol

MGC:73031

Previous Names

• FR_5351 (1)

Type

cDNA

Location

Unmapped

Genome Resources

None

Species

Danio rerio

Library

Zebrafish adult retina cDNA

Digest

Insert Size

2143

Cloning Site

EcoRI-SalI

Vector Type

Plasmid

Vector

pBluescript SK-

Polymerase

T7 RNA polymerase

PCR Amplification

Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' AAC AGC TAT GAC CAT G 3'
5' GTA AAA CGA CGG CCA GT 3'

Suppliers

None

Quality

( Simple to use, intense expression pattern restricted to a few structures )

Protocol

Thisse in situ hybridization protocol

Note

None