MGC:73181

ID

ZDB-CDNA-040425-2644

Name

MGC:73181

Symbol

MGC:73181

Previous Names

• FR133 (1)

Type

cDNA

Location

Unmapped

Genome Resources

None

Species

Danio rerio

Library

Zebrafish adult retina cDNA

Digest

Insert Size

1121

Cloning Site

EcoRI-SalI

Vector Type

Plasmid

Vector

pBluescript SK-

Polymerase

T7 RNA polymerase

PCR Amplification

Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' AAC AGC TAT GAC CAT G 3'
5' GTA AAA CGA CGG CCA GT 3'

Suppliers

None

Protocol

Thisse in situ hybridization protocol

Note

None