cDNA Name: |
MGC:63964
|
Sequence Ontology ID : |
SO:0000756 |
Previous Name:
|
FR230 (1)
|
Attributions for Alias: {{control.newAlias}}
Delete Alias:
(Including Attributions)
|
Species: |
Danio rerio |
Library: |
NCI_CGAP_ZKid1 |
Digest: |
|
Insert Size: |
1412 |
Cloning Site: |
NotI-SmaI |
Vector Type: |
phagemid |
Vector: |
pCMV-SPORT6.1 |
Polymerase: |
T7 RNA polymerase |
PCR Amplification: |
Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min. 5' AAC AGC TAT GAC CAT G 3' 5' GTA AAA CGA CGG CCA GT 3' |
Source: |
|
Protocol: |
Thisse in situ hybridization protocol
|
Location: |
Unmapped
|